TPCI - Materials Syntesis and Physical Chemistry

We take advantage of the built-in functionalities of the copolymers we synthesize in order to construct complex (bio)hybrid nanostructures and nanomaterials, able to be utilized in a number of nanotechnological applications by providing proof-of-concept studies. We mainly make use of electrostatic co-assembly, and hydrophobic interactions, between synthetic copolymers and other building blocks available, like nucleic acids (DNAs, RNAs), proteins/peptides, hydrophobic drugs, lipids, surfactants, natural polymers, inorganic magnetic/plasmonic nanoparticles and carbon nanomaterials, toward the formation of tailor-made hierarchical, environmentally responsive and (bio)functional nanoconstructs, nanocarriers and nanocontainers.

We pursue utilization of these nanostructures in gene and drug delivery schemes for therapy, bio-imaging, theranostics, (bio)sensing, water purification technologies and for advanced solid polymer electrolyte systems.

Block copolymer and polyelectrolyte complexes

Murmiliuk, S. K. Filippov, O. Rud, P. Kosovan, Z. Tosner, A. Radulescu, A. Skandalis, S. Pispas, M. Slouf, M. Stepanek, "Reversible multilayered vesicle-like structures with fluid hydrophobic and interpolyelectrolyte layers", J. Colloid Int. Sci. 2021, 599, 313.
DOI: 10.1016/j.jcis.2021.04.050
A. Fanova, I. Davidovich, Y. Talmon, A. Skandalis, S. Pispas, M. Stepanek, "Modification of the Co-assembly Behavior of Double-Hydrophilic Block Polyelectrolytes by Hydrophobic Terminal Groups: Ordered Nanostructures with Interpolyelectrolyte Complex Domains", ACS Appl. Polym. Mater. 2021, 3, 1956.
DOI: 10.1021/acsapm.1c00033
R. K. Raya, M. Stepanek, Z. Limpouchova, K. Prochazka, M. Svoboda, M. Lisal, E. Pavlova, A. Skandalis, S. Pispas, "Onion micelles with an interpolyelectrolyte complex middle layer: Experimental motivation and computer study", Macromolecules 2020, 53, 6780.
DOI: 10.1021/acs.macromol.0c00560
Fanova, K. Sindelka, M. Uchman, Z. Limpouchova, S. K. Filippov, S. Pispas, K. Prochazka, M. Stepanek, "Coassembly of poly(N-isopropylacrylamide) with dodecyl and carboxyl terminal groups with cationic surfactant: critical comparison of experimental and simulation data", Macromolecules 2018, 51, 7295.
DOI: 10.1021/acs.macromol.8b01161
Murmiliuk, P. Matejicek, S. K. Filippov, M. Janata, M. Slouf, S. Pispas, M. Stepanek, "Formation of core/corona nanoparticles with interpolyelectrolyte complex cores in aqueous solution: insight into chain dynamics in the complex from fluorescence quenching", Soft Matter 2018, 14, 7578.
DOI: 10.1039/c8sm01174e
M. Uchman. J. Hajduova, E. Vlassi, S. Pispas, M-S. Appavou, M. Stepanek, "Self- and co-assembly of amphiphilic gradient polyelectrolyte in aqueous solution: Interaction with oppositely charged ionic surfactant", Eur. Polym. J. 2015, 73, 212.
DOI: 10.1016/j.eurpolmj.2015.10.015
M. Uchman, S. Pispas, L. Kovacik and M. Stepanek, "Morphologically tunable coassembly of double hydrophilic block polyelectrolyte with oppositely charged fluorosurfactant", Macromolecules 2014, 47, 7081.
DOI: 10.1021/ma500622a
J. Hajduova, K. Prochazka, M. Slouf, B. Angelov, G. Mountrichas, S. Pispas, M. Stepanek, "Polyelectrolyte-surfactant complexes of poly[3,5-bis(dimethylaminomethyl)-4-hydroxystyrene]-block-poly(ethylene oxide) and sodium dodecyl sulfate: Anomalous self-assembly behavior", Langmuir 2013, 29, 5443.
DOI: 10.1021/la400583z

Block copolymer/DNA and protein complexes, and biohybrid nanostructures

A. Skandalis, D. Selianitis, S. Pispas, "PnBA-b-PNIPAM-b-PDMAEA thermo-responsive triblock terpolymers and their quaternized analogs as gene and drug delivery vectors", Polymers 2021, 13, 2361.
DOI: 10.3390/polym13142361
A. Chroni, T. Mavromoustakos, S. Pispas, "Nano-assemblies from amphiphilic PnBA-b-POEGA copolymers as drug nanocarriers", Polymers 2021, 13, 1164.
DOI: 10.3390/polym13071164
E. Haladjova, V. Chrysostomou, M. Petrova, I. Ugrinova, S. Pispas, S. Rangelov, "Physicochemical Properties and Biological Performance of Polymethacrylate Based Gene Delivery Vector Systems: Influence of Amino Functionalities", Macromol. Biosci. 2021, 21, 2000352.
DOI: 10.1002/mabi.202000352
Skandalis, A. Murmiliuk, M. Stepanek, S. Pispas, "Physicochemical evaluation of insulin complexes with QPDMAEMA-b-PLMA-b-POEGMA cationic amphiphlic triblock terpolymer micelles", Polymers 2020, 12, 309.
DOI: 10.3390/polym12020309
T. Sendoukas, S. Pispas, "Poly(2-(dimethylamino)ethyl methacrylate)-b-poly(hydroxypropyl methacrylate) copolymers/bovine serum albumin complexes in aqueous solutions", J. Polym. Sci. 2020, 58, 1241.
DOI: 10.1002/pol.20200065
Α. Chroni, S. Pispas, "Hydrophilic/hydrophobic modifications of a PnBA-b-PDMAEA copolymer and complexation behaviour with short DNA", Eur. Polym. J. 2020, 129, 109636.
DOI: 10.1016/j.eurpolymj.2020.109636
M. Vlachou, A. Siamidi, E. Goula, P. Georgas, N. Pippa, V. Karalis, T. Sentoukas, S. Pispas, "Probing the release of the chronobiotic hormone melatonin from hybrid calcium alginate hydrogel beads", Acta Pharm. 2020, 70, 527.
DOI: 10.2478/acph-2020-0037
Skandalis, M. Uchman, M. Stepanek, S. Kereiche, S. Pispas, "Complexation of DNA with QPDMAEMA-b-PLMA-b-POEGMA cationic triblock terpolymer micelles", Macromolecules 2020, 53, 5747.
DOI: 10.1021/acs.macromol.0c00388
M. Kafetzi, S. Pispas, X. Bao, P. Yao, "Amphiphilic QP(DMAEMA-co-LMA)-b-POEGMA random-block terpolymers as nanocarriers for insulin", Biomedicines 2020, 8, 392.
DOI: 10.3390/biomedicines8100392
T. Sentoukas, S. Pispas, "Poly(2-[dimethylamino]ethyl methacrylate)-b-poly(hydroxypropyl methacrylate)/DNA polyplexes in aqueous solutions", J. Polym. Sci. 2020, 58, 2335.
DOI: 10.1002/pol.20200375
Chroni, T. Mavromoustakos, S. Pispas, "Biocompatible PEO-b-PCL nanosized micelles as drug carriers: Structure and drug-polymer interactions", Nanomaterials 2020, 10, 1872.
DOI: 10.3390/nano10091872
E. Haladjova, G. Mountrichas, S. Pispas, S. Rangelov, "Determination of intimate composition of theranostic polyplexes based on (co)polymers of poly(vinyl benzyl trimethylammonium chloride", Macromol. Chem. Phys. 2018, 219, 1700428.
DOI: 10.1002/macp.2-1700428
K. Kamenova, E. Haladjova, G. Grancharov, M. Kyulavska, V. Tzankova, D. Aluani, K. Yoncheva, S. Pispas, P. Petrov, "Co-assembly of block copolymers as a tool for developing novel micellar carriers of insulin for controlled drug delivery", Eur. Polym. J. 2018, 104, 1.
DOI: 10.1016/j.eurpolymj.2018.04.039
S. K. Filippov, A. Papagiannopoulos, A. Riabtseva, S. Pispas, "Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC", Colloid Polym. Sci. 2018, 296, 1183.
DOI: 10.1007/s00396-018-4329-4
Petropoulou, T. J. Gibson, E. Themistou, S. Pispas, and C. Riziotis, "Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection", Mater. Chem. Phys. 2018, 216, 421.
DOI: 10.1016/j.matchemphys.2018.06.027
Papagiannopoulos, E. Vlassi, S. Pispas, J. E. Houston, "Association and internal morphology of self-assembled HPPhOx/BSA hybrid nanoparticles in aqueous solutions", J. Phys. Chem. B 2018, 122, 7426.
DOI: 10.1021/acs.jpcb.8b04364
E. Vlassi, A. Papagiannopoulos, S. Pispas, "Amphiphilic poly(2-oxazoline) copolymers as self-assembled carriers for drug delivery applications", Eur. Polym. J. 2017, 88, 516. (Feature Article)
DOI: 10.1016/j.eurpolymj.2016.10.034
B. Lou, N. Beztsinna, G. Mountrichas, J. B. van den Dikkenberg, S. Pispas, W. E. Hennink, "Small nanosized poly(vinyl benzyl trimethylammonium chloride) based polyplexes for siRNA delivery", Int. J. Pharm. 2017, 525, 388.
DOI: 10.1016/j.ijpharm.2017.03.036
Papagiannopoulos, E. Vlassi, S. Pispas, C. J. Jafta, "Tuning the solution organization of cationic polymers through interactions with bovine serum albumin", Phys. Chem. Chem. Phys. 2017, 19, 18471.
DOI: 10.1039/C7CP02704D
E. Vlassi, A. Papagiannopoulos, S. Pispas, "Self-assembly of poly(ethylene glycol-b-phenyl oxazoline) diblock copolymers in aqueous media and their interactions with proteins", Colloid Polym. Sci. 2017, 295, 1359. (invited article)
DOI: 10.1007/s00396-017-4076-y
N. Pippa, A. Meristoudi, S. Pispas, C. Demetzos, "Lysozyme complexes with thermo- and pH-responsive PNIPAM-b-PAA block copolymers", J. Nanopart. Res. 2017, 19, 76.
DOI: 10.1007/s11051-017-3782-1
N. Pippa, G. Mountrichas, S. Pispas, C. Demetzos, G. Sivolapenko, "Preparation and physicochemical characterization of polyelectrolyte complexes incorporating antitumor peptide", J. Nanosci. Nanotech. 2017, 17, 4901.
DOI: 10.1166/jnn.2017.13738
Papagiannopoulos, A. Meristoudi, K. Hong, S. Pispas, "Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme", Polymer 2016, 83, 111.
DOI: 10.1016/j.polymer.2015.12.023
E. Haladjova, G. Mountrichas, S. Pispas, S. Rangelov, "Poly(vinyl benzyl trimethylammonium chloride) homo and block copolymers complexation with DNA", J. Phys. Chem. B 2016, 120, 2586.
DOI: 10.1021/acs.jpcb.5b12477
M. Karayianni, R. Radeva, N. Koseva, S. Pispas, "Electrostatic complexation of a double hydrophilic block polyelectrolyte and proteins of different molecular shape", J. Polym. Sci., Part B: Polym. Phys. 2016, 54, 1515.
DOI: 10.1002/polb.24047
M. Karayianni, V. Gancheva, S. Pispas, P. Petrov, "Complex formation between lysozyme and stabilized micelles with a mixed poly(ethylene oxide)/poly(acrylic acid) shell", J. Phys. Chem. B 2016, 120, 2625.
DOI: 10.1021/acs.jpcb.6b00550
A. Papagiannopoulos, A. Meristoudi, S. Pispas, U. Keiderling, "Thermoresponsive behavior of micellar aggregates from end-functionalized PnBA-b-PNIPAM-COOH block copolymers and their complexes with lysozyme", Soft Matter 2016, 12, 6547.
DOI: 10.1039/c6sm00976j
Papagiannopoulos, A. Meristoudi, S. Pispas, A. Radulescu, "Micelles from HOOC-PnBA-b-PAA-C₁₂H₂₅ diblock amphiphilic polyelectrolytes as protein nanocarriers", Biomacromolecules 2016, 17, 3816.
DOI: 10.1021/acs.biomac.6b01408
A. Papagiannopoulos, S. Pispas, "Mixed protein/polymer nanostructures at interfaces", in Advanced Materials Interfaces, Advanced Materials Book Series, Eds. A. Tiwari, H. K. Patra, X. Wang, Wiley-Scrivener Publishing LLC, USA, 2016. (invited).
A. Papagiannopoulos, A. Christoulaki, N. Spiliopoulos, A. Vradis, C. Toprakcioglu, S. Pispas, "Complexation of lysozyme with adsorbed PtBS-b-SCPI block polyelectrolyte micelles on silver surface", Langmuir 2015, 31, 685.
DOI: 10.1021/la504873h
E. Vlassi, S. Pispas, "Imidazolium quaternized polymers based on poly(chloromethyl styrene) and their complexes with FBS proteins and DNA", Macromol. Chem. Phys. 2015, 216, 1718.
DOI: 10.1002/macp.201500162
E. Vlassi, S. Pispas, "Solution behavior of hydrolysed gradient methyl/phenyl oxazoline copolymers and complexation with DNA", Macromol. Chem. Phys. 2015, 216, 873.
DOI: 10.1002/macp.201400552
El Sachat, A. Meristoudi, S. Pispas, C. Riziotis, "Assessment of block and random copolymer overlayers on polymer optical fibers toward protein detection through electrostatic interaction", J. Polym. Sci. Part A: Polym. Phys. 2015, 53, 327.
DOI: 10.1002/polb.23632
N. Pippa, S. Pispas, C. Demetzos, "Delivery nanoparticle platform of liposomes - incorporated dendrimers: physicochemical, and thermotropic characterization", Advanced Science, Engineering and Medicine, 2015, 6, 805.
DOI: 10.1166/asem.2015.1761
N. Pippa, M. Karayianni, S. Pispas, C. Demetzos, "Complexation of cationic-neutral block polyelectrolyte with insulin and in vitro release studies", Int. J. Pharm. 2015, 491,136.
DOI: 10.1016/j.ijpharm.2015.06.013
N. Pippa, R. Kalinova, I. Dimitrov, S. Pispas, C. Demetzos, "Insulin/poly(ethylene glycol)-block-poly(L-lysine) complexes: Physicochemical properties and protein encapsulation", J. Phys. Chem. B. 2015, 119, 6813.
DOI: 10.1021/acs.jpcb.5b01664
N. Pippa, A. Meristoudi, S. Pispas, C. Demetzos, "Temperature-dependent drug release from DPPC:C₁₂H₂₅-PNIPAM-COOH liposomes: control of the drug loading / release by modulation of the nanocarriers' components", Int. J. Pharm. 2015, 485, 374.
DOI: 10.1016/j.ijpharm.2015.03.014
N. Pippa, S. Pispas, C. Demetzos, "The metastable phases as modulators of biophysical behavior of liposomal membranes: The role of biomolecular sculpture of polymeric guest", J. Therm. Anal. Calorim. 2015, 120, 937.
DOI: 10.1007/s10973-014-4116-5
Jelezova, E. Drakalska, D. Momekova, N. Shalimova, G. Momekov, S. Konstantinov, S. Rangelov, S. Pispas, "Curcumin loaded pH-sensitive hybrid lipid/block copolymer nanosized drug delivery systems", Eur. J. Pharm. Sci. 2015, 78, 67.
DOI: 10.1016/j.ejps.2015.07.005
V. Sereti, M. Zoumpanioti, V. Papadimitriou, S. Pispas, A. Xenakis, "Biocolloids based on amphiphilic block copolymers as a medium for enzyme encapsulation", J. Phys, Chem. B 2014, 118, 9808.
DOI: 10.1021/jp504449y

Block copolymer/lipid hybrid assemblies

N. Naziris, N. Pippa, A. Skandalis, K. Milowska, L. Balcerzak, S. Pispas, M. Bryszewska, C. Demetzos, "Thermoresponsive chimeric nanocarriers as drug delivery systems", Colloids Surf. B 2021, 208, 112141.
DOI: 10.1016/j.colsurfb.2021.112141
A. Forys, M. Chountoulesi, B. Mendrek, T. Konieczny, T. Sentoukas, M. Godzierz, A. Kordyka, C. Demetzos, S. Pispas, B. Trzebicka, "The influence of hydrophobic blocks of PEO-containing copolymers on glyceryl monooleate lyotropic liquid crystalline nanoparticles for drug delivery", Polymers 2021, 13, 2607.
DOI: 10.3390/polym13162607
N. Naziris, N. Pippa, E. Sereti, V. Chrysostomou, M. Kędzierska, J. Kajdanek, M. Ionov, K. Milowska, L. Balcerzak, S. Garofalo, C. Limatola, S. Pispas, K. Dimas, M. Bryszewska, C. Demetzos, "Chimeric stimuli-responsive liposomes as nanocarriers for the delivery of the anti-glioma agent TRAM-34", Int. J. Mol. Sci. 2021, 22, 6271.
DOI: 10.3390/ijms22126271
T. Sentoukas, C. Demetzos, S. Pispas, "Chimeric liposomes incorporating functional copolymers: preparation and pH/thermo-responsive behaviour in aqueous solutions", J. Liposome Res. 2021, 31, 279.
DOI: 10.1080/08982104.2020.1806873
A. Papagiannopoulos, N. Pippa, C. Demetzos, S. Pispas, A. Radulescu, "Formation of uni-lamellar vesicles in mixtures of DPPC with PEO-b-PCL amphiphilic diblock copolymers", Polymers 2021, 23, 1.
DOI: 10.3390/polym13010004
A. Papagiannopoulos, N. Pippa, C. Demetzos, S. Pispas, A. Radulescu, "Lamellarity and size distributions in mixed DPPC/amphiphilic poly(2-oxazoline) gradient copolymer vesicles and their temperature response", Chem. Phys. Lipids 2021, 234, 105008.
DOI: 10.1016/j.chemphyslip.2020.105008
M. Chountoulesi, D. R. Perinelli, A. Forys, G. Bonacucina, B. Trzebicka, S. Pispas, C. Demetzos, "Liquid crystalline nanoparticles for drug delivery: The role of gradient and block copolymers on the morphology, internal organisation and release profile", Eur. J. Pharm. Biopharm. 2021, 158, 21.
DOI: 10.1016/j.ejpb.2020.08.008
N. Naziris, F. Saitta, V. Chrysostomou, M. Libera, B. Trzebicka, D. Fessas, S. Pispas, C. Demetzos, "pH-responsive chimeric liposomes: From nanotechnology to biological assessment", Int. J. Pharm. 2020, 574, 118849.
DOI: 10.1016/j.ijpharm.2019.118849
M. Chountoulesi, D. R. Perinelli, N. Pippa, V. Chrysostomou, A. Forys, L. Otulakowski, G. Bonacucina, B. Trzebicka, S. Pispas, C. Demetzos, "Physicochemical, morphological and thermal evaluation of lyotropic lipidic liquid crystalline nanoparticles: The effect of stimuli-responsive polymeric stabilizer", Colloids & Surfaces A 2020, 595, 124678.
DOI: 10.1016/j.colsurfa.2020.124678
N. Naziris, A. Skandalis, A. Forys, B. Trzebicka, S. Pispas, C. Demetzos, "A thermal analysis and physicochemical study on thermoresponsive chimeric liposomal nanosystems", J. Therm. Anal. Cal. 2020, 141, 751.
DOI: 10.1007/s10973-019-09049-z
N. Pippa, N. Naziris, D. Stellas, C. Massala, K. Zouliati, S. Pispas, C. Demetzos, A. Forys, A. Marcinkowski, "PEO-b-PCL grafted niosomes: The cooperativity of amphiphilic components and their properties in vitro and in vivo", Colloids Surf. B 2019, 177, 338.
DOI: 10.1016/j.colsufb.201901.036
M. Chountoulesi, N. Pippa, V. Chrysostomou, S. Pispas, E. D. Chrysina, A. Forys, L. Otulakowski, B. Trzebicka, C. Demetzos, "Stimuli-responsive lyotropic liquid crystalline nanosystems with incorporated poly(2-dimethylamino ethyl methacrylate)-b-poly(lauryl methacrylate) amphiphilic block copolymer", Polymers 2019, 11, 1400.
DOI: 10.3390/polym11091400
N. Pippa, D. R. Perinelli, S. Pispas, G. Bocacucina, C. Demetzos, A. Forys, and B. Trzebicka, "Studying the colloidal behavior of chimeric liposomes by cryo-TEM, micro-differential scanning calorimetry and high-resolution ultrasound spectroscopy", Colloids Surf. A 2018, 555, 539.
DOI: 10.1016/j.colsurfa.2018.07.025
N. Naziris, N. Pippa, D. Stellas, V. Chrysostomou, S. Pispas, C. Demetzos, M. Libera, B. Trzebicka, "Development and evaluation of stimuli-responsive chimeric nanostructures", AAPS PharmSciTech 2018, 19, 2971.
DOI: 10.1208/s12249-018-1112-2
M. Chountoulesi, N. Pippa, S. Pispas, E. D. Chrysina, A. Forys, B. Trzebicka, C. Demetzos, "Cubic lyotropic liquid crystals as drug delivery carriers: Physicochemical and morphological studies", Int. J. Pharm. 2018, 550, 57.
DOI: 10.1016/j.ijpharm.2018.08.003
K. Zouliati, C. Massala, N. Pippa, N. Naziris, S. Pispas, C. Demetzos, "Influence of lipid's main transition temperature on the stability of chimeric liposomal systems", Current Nanomedicine 2018, 8, 1.
DOI: 10.2174/2405461503666180912095425
Tzani, N. Naziris, N. Pippa, S. Pispas, C. Demetzos, "Innovative drug nanocarriers by incorporating thermoresponsive polymer in phospholipid bilayer", Glob. Drugs Therap. 2017, 2, 1.
DOI: 10.15761/GDT.1000133
C. Demetzos, N.Pippa, S. Pispas, "Advances and perspectives in cancer nanotherapy: The added value of nanocarriers.", Curr. Nanomed. 2017, 7, 170. (invited review article)
DOI: 10.2174/2468187307666170411101920
N. Naziris, N. Pippa, A. Meristoudi, S. Pispas, C. Demetzos, "Design and development of pH-responsive HSPC:C₁₂H₂₅-PAA chimeric liposomes", J. Liposome Res. 2017, 27, 108.
DOI: 10.3109/08982104.2016.1166512
Kyrili, M. Chountoulesi, N. Pippa, A. Meristoudi, S. Pispas, C. Demetzos, "Design and development of pH sensitive liposomes by evaluating the thermotropic behavior of their chimeric bilayers", J. Therm. Anal. Calorim. 2017, 127, 1381.
DOI: 10.1007/s10973-016-6069-3
M. Chountoulesi, A. Kyrili, N. Pippa, A. Meristoudi, S. Pispas, C. Demetzos, "The modulation of physicochemical characterization of innovative liposomal platforms: the role of the grafted themoresponsive polymers", Pharm. Develop. Techn. 2017, 22, 330.
DOI: 10.3109/10837450.2015.1121497
N. Pippa, A. Skandalis, S. Pispas, C. Demetzos, M. Libera, A. Marcinkowski, B. Trzebicka, "Mixed lipid/polymer nanostructures: from advanced materials to drug delivery systems", Adv. Mater. Lett. 2017, 8, 428.
DOI: 10.5185/amlett.2017.1414
N. Naziris, N. Pippa, V. Chrysostomou, S. Pispas, C. Demetzos, M. Libera, B. Trzebicka, "Morphological diversity of block copolymer/lipid chimeric nanostructures", J. Nanopart. Res. 2017, 19, Art. 347.
DOI: 10.1007/s11051-017-4021-5
N. Pippa, D. Stellas, A. Skandalis, S. Pispas, C. Demetzos, M. Libera, A. Marcinkowski, B. Trzebicka, "Chimeric lipid/block copolymer nanovesicles: physicochemical and bio-compatibility evaluation", Eur. J. Pharm. Biopharm. 2016, 107, 295.
DOI: 10.1016/j.ejpb.2016.08.003
N. Pippa, S. Pispas, C. Demetzos, "Polymer self-assembled nanostructures as innovative drug nanocarrier platforms", Curr. Pharm. Des. 2016, 22, 2788. (invited review article)
DOI: 10.2174/1381612822666160217141232
Kolman, N. Pippa, A. Meristoudi, S. Pispas and C. Demetzos, "A dual stimuli-responsive polymer into phospholipid membranes: a thermotropic approach", J. Therm. Anal. Calorim. 2016, 123, 2257.
DOI: 10.1007/s10973-015-5080-4
N. Pippa, M. Chountoulesi, A. Kyrili, A. Meristoudi, S. Pispas, C. Demetzos, "Calorimetric study on pH-responsive block copolymer grafted lipid bilayers: Rational design and development of liposomes", J. Liposome Res. 2016, 26, 211.
DOI: 10.3109/08982104.2015.1076464
N. Pippa, S. Pispas, C. Demetzos, "Delivery nanoparticle platform of liposomes - incorporated dendrimers: physicochemical, and thermotropic characterization", Advanced Science, Engineering and Medicine, 2015, 6, 805.
DOI: 10.1166/asem.2015.1761
N. Pippa, M. Mariaki, S. Pispas, C. Demetzos, "Preparation, development and in vitro release evaluation of Amphotericin B-loaded amphiphilic block copolymer vectors", Int. J. Pharm. 2014, 473, 80.
DOI: 10.1016/j.ijpharm.2014.07.001
N. Pippa, A. Dokoumetzidis, S. Pispas, C. Demetzos, "The interplay between the rate of the release from polymer grafted liposomes and their fractal morphology", Int. J. Pharm. 2014, 465, 63.
DOI: 10.1016/j.ijpharm.2014.02.010
N. Pippa, S. Pispas, C. Demetzos, "The imaging and the fractal metrology of chimeric liposomal Drug Delivery nano Systems: The role of macromolecular architecture of polymeric guest", J. Liposome Res. 2014, 24, 223.
DOI: 10.3109/08982104.2014.891232
N. Pippa, E. Kaditi, S. Pispas, C. Demetzos, "Gradient block copolymer structures as drug nanocarriers", Adv. Sci. Eng. Med. 2014, 6, 642.
DOI: 10.1166/asem.2014.1554
N. Pippa, E. Deli, E. Mentzali, S. Pispas, C. Demetzos, "PEO-b-PCL grafted DPPC liposomes: physicochemical characterization and stability studies of novel bio-inspired advanced Drug Delivery nano Systems (aDDnSs)", J. Nanosci. Nanotech. 2014, 14, 5676.
DOI: 10.1166/jnn.2014.8869
N. Pippa, K. Gardikis, S. Pispas, C. Demetzos, "The physicochemical/thermodynamic balance of advanced drug liposomal delivery systems", J. Therm. Anal. Calorim. 2014, 116, 99.
DOI: 10.1007/s10973-013-3406-7
N. Pippa, E. Kaditi, S. Pispas, C. Demetzos, "PEO-b-PCL-DPPC chimeric nanocarriers: self-assembly aspects in aqueous and biological media and drug incorporation", Soft Matter 2013, 9, 4073.
DOI: 10.1039/c3sm27447k
N. Pippa, E. Kaditi, S. Pispas, C. Demetzos, "DPPC/poly(2-methyl-2-oxazoline)-grad-poly(2-phenyl-2-oxazoline) chimeric nanostructures as potential drug nanocarriers", J. Nanopart. Res. 2013, 15, 1685.
DOI: 10.1007/s11051-013-1685-3
M. Hadjidemetriou, N. Pippa, S. Pispas, C. Demetzos, "Incorporation of dimethoxycurcumin into charged liposomes and the formation kinetics of fractal aggregates of uncharged vectors", J. Liposome Res. 2013, 23, 94.
DOI: 10.3109/08982104.2012.747534
N. Pippa, M. Merkouraki, S. Pispas, C. Demetzos, "DPPC:MPOx chimeric advanced drug delivery nano systems (chi-aDDnSs): Physicochemical and structural characterization, stability and drug release studies", Int. J. Pharm. 2013, 450, 1.
DOI: 10.1016/j.ijpharm.2013.03.052
N. Pippa, F. Psarommati, S. Pispas, C. Demetzos, "The shape/morphology balance: a study of stealth liposomes via fractal analysis and drug encapsulation", Phar. Res. 2013, 30, 2385.
DOI: 10.1007/s11095-013-1082-8
N. Pippa, S. Pispas, C. Demetzos, G. Sivolapenko, "Advanced nanocarriers for an antitumor peptide", J. Nanopart. Res. 2013, 15, 2062.
DOI: 10.1007/s11051-013-2062-y

Block copolymer/inorganic nanoparticles hybrid nanostructures

M. Kafetzi, K. B. L. Borchert, C. Steinbach, D. Schwarz, S. Pispas, S. Schwarz, "Thermoresponsive PNIPAM-b-PAA block copolymers as "smart" adsorbents of Cu(II) for water restore treatments", Colloids Surf. A: Phys. Eng. Asp. 2021, 614, 126049.
DOI: 10.1016/j.colsurfa.2020.126049
E. Vlassi, A. Papagiannopoulos, A. Sergides, S. Pispas, "Block copolymer nanosystems encapsulating magnetic nanoparticles and drug", J. Nanoscience Nanotechnology 2020, 20, 3981.
DOI: 10.1166/jnn.2020.176382
Chroni, A. Forys, B. Trzebicka, A. Alemayehu, V. Tyrpekl, S. Pispas, "Poly[oligo(ethylene glycol) methacrylate]-b-poly[(vinyl benzyl trimethylammonium chloride)] based multifunctional hybrid nanostructures encapsulating magnetic nanoparticles and DNA", Polymers 2020, 12, 1283.
DOI: 10.3390/polym12061283
Kagkoura, T. Sentoukas, Y. Nakanishi, H. Shinohara, S. Pispas, N. Tagmatarchis, "Bottom-up microwave-assisted preparation of poly(methacrylic acid)-MoS₂ hybrid material", Chem. Phys. Lett. 2019, 716, 1.
DOI: 10.1016/j.cplett.2018.12.002
Skandalis, A. Sergides, A. Bakandritsos, S. Pispas, "PLMA-b-POEGMA amphiphilic block copolymers as nanocarriers for the encapsulation of magnetic nanoparticles and indomethacin", Polymers 2018, 10, 14.
DOI: 10.3390/polym10010014
Papagiannopoulos, G. Mousdis, S. Pispas, "Au nanoparticle-corona loaded polystyrene-b-quaternized poly(2-vinylpyridine) micelles and their interaction with DNA", Macromol. Chem. Phys. 2017, 218, 1600439.
DOI: 10.1002/macp.201600439
T. Skaltsas, M. Goulielmaki, A. Pintzas, S. Pispas, and N. Tagmatarchis, "Carbon quantum dots/block copolymer ensembles for metal-ion sensing and bioimaging", J. Mater. Chem. B 2017, 5, 5397.
DOI: 10.1039/c7tb01352c
M. Vasileiadis, I. Koutselas, S. Pispas, N. A. Vainos, "Design and evaluation of polymer matrices for the encapsulation of CdSe/ZnS quantum dots in photonic nanocomposite films", J. Polym. Sci., Part B: Polym. Phys. 2016, 54, 552.
DOI: 10.1002/polb.23921
G. Zardalidis, A. Pipertzis, G. Mountrichas, S. Pispas, M. Mezger, G. Floudas, "Effect of polymer architecture on the ionic conductivity. Densely grafted poly(ethylene oxide) brushes doped with LiTf", Macromolecules 2016, 49, 2679.
DOI: 10.1021/acs.macromol.6b00290
E. Vlassi, G. A. Mousdis, S. Pispas, "Nickel dithiolene complexes encapsulated in biocompatible amphiphilic diblock copolymer nanoparticles", J. Polym. Sci., Part B: Polym. Phys. 2016, 54, 2507.
DOI: 10.1002/polb.24247
M. Mihai, G. Mountrichas, S. Pispas, B. C. Simionescu, "Effects of PHOS-b-PMAA additive and pH on the formation of calcium carbonate composite microparticles", Revue Roumanie de Chemie 2016, 61, 657.
G. Zardalidis, E. F. Ioannou, K. D. Gatsouli, S. Pispas, E. I. Kamitsos, G. Floudas, "Ionic conductivity and self-assembly in poly(isoprene-b-ethylene oxide) electrolytes doped with LiTF and EMITf", Macromolecules 2015, 48, 1473.
DOI: 10.1021/acs.macromol.5b00089
E. Haladjova, S. Rangelov, M. Geisler, S. Boye, A. Lebeler, G. Mountrichas, S. Pispas, "Asymmetric flow field-flow fractionation investigation of magnetopolyplexes", Macromol. Chem. Phys. 2015, 216, 1862.
DOI: 10.1002/macp.201500177
J. Hajduova, M. Uchman, I. Safarik, M. Safarikova, M. Slouf, S. Pispas, M. Stepanek, "Aggregation of superparamagnetic iron oxide nanoparticles in dilute aqueous dispersions: Effect of coating by double-hydrophilic block polyelectrolyte". Colloids Surf. A: Physicochemical Eng. Aspects 2015, 483, 1.
DOI: 10.1016/j.colsurfa.2015.07.008
G. Zardalidis, K. Gatsouli, S. Pispas, M. Mezger, G. Floudas, "Ionic conductivity, self-assembly, and viscoelasticity in poly(styrene-b-ethylene oxide) electrolytes doped with LiTf", Macromolecules 2015, 48, 7164.
DOI: 10.1021/acs.macromol.5b1596
T. Skaltsas, N. Tagmatarchis, S. Pispas, "Non-covalent graphene/polymer functional materials", Curr. Org. Chem. 2015, 19, 1800. (invited review article)
WOS: 000360379700006
T. Skaltsas, G. Mountrichas, S. Zhao, H. Shinohara, N. Tagmatarchis, S. Pispas, "Single-step functionalization and exfoliation of graphene with polymers under mild conditions", Chemistry: A European Journal 2015, 21, 18841.
DOI: 10.1002/chem.201500278
Matuszewska, M. Uchman, A. Adamczyk-Wozniak, A. Sporzynski, S. Pispas, L. Kovacik, and M. Stepanek, "Glucose-responsive hybrid nanoassemblies in aqueous solutions: Ordered phenylboronic acid within intermixed poly(4-hydroxystyrene)-block-poly(ethylene oxide) block copolymer", Biomacromolecules 2015, 16, 3731.
DOI: 10.1021/acs.biomac.5b01325
T. Skaltsas, S. Pispas, N. Tagmatarchis, "Non-covalent nanodiamont-polymer dispersions and electrostatic immobilization of bovine serum albumin protein", Materials Research Express 2015, 2, 115005.
DOI: 10.1088/2053-1591/2/11/115005
E. Haladjova, S. Rangelov, Ch. B. Tsvetanov, V. Posheva, E. Peycheva, V. Maximova, D. Momekova, G. Mountrichas, S. Pispas, A. Bakandritsos, "Enhanced gene expression promoted by hybrid magnetic/cationic block copolymer micelles", Langmuir 2014, 30, 8193.
DOI: 10.1021/la501402q
G. Mountrichas, S. Pispas, E. I. Kamitsos, "Effect of temperature on the direct synthesis of gold nanoparticles mediated by poly(dimethylaminoethyl methacrylate) homopolymer", J. Phys. Chem. C 2014, 118, 22.
DOI: 10.1021/jp505725v
T. Skaltsas, N. Karousis, S. Pispas, N. Tagmatarchis, "Photocatalytic applications with CdS/block copolymer/exfoliated graphene nanoensembles: hydrogen generation and degradation of Rhodamine B", Nanotechnology 2014, 25, 445404.
DOI: 10.1088/0957-4484/25/44/445404
G. Zardalidis, E. Ioannou, S. Pispas, G. Floudas, "Relating structure, viscoelasticity, and local mobility to conductivity in PEO/LiTf electrolytes", Macromolecules 2013, 46, 2705.
DOI: 10.1021/ma400266w
M. Mihai, G. Mountrichas, S. Pispas, I. Stoica, M. Aflori, M.A. der Landwehr, I. Neda, S. Schwarz, "Calcium carbonate microparticle templates using a PHOS-b-PMAA double hydrophilic copolymer", J. Appl. Cryst. 2013, 46, 1455.
DOI: 10.1107/S0021889813020918