Biomimetics & Nanobiotechnology Dr. Aris Xenakis | Researcher Emeritus
Aims
The Biomimetics and Nanobiotechnology group deals two main axes
Development and structural analysis of systems for the encapsulation of bioactive compounds
The expertise of the group is the development and characterization of biocompatible nanodispersions (micro- and nano-emulsions) as well as gels (organo- and hydro-gels) for the encapsulation, protection and controlled release of bioactive compounds. A variety of nanocarriers have been developed with high or low energy techniques, involving biocompatible and biodegradable surfactants, oils of natural origin as well as biopolymers. The nanocarriers are physiochemically characterized with advanced techniques, in vitro evaluated via cytotoxicity using specific cell lines and additionally evaluated towards their ability to release the encapsulated bioactive compounds.
Structural and functional studies of immobilised enzymes - Biocatalysis
The second main axe involves the immobilisation of enzymes in nanodispersions that mimic the biological membranes, or in solid / semi-solid carriers. Structural and morphological analysis is conducted for the developed systems as well as the incorporated compounds. The group studies the use of the immobilized enzymes for the production of high added value products such as phenolic or fatty acid esters or other compounds of industrial interest (i.e. antioxidants, flavour improving additives, lubricants, edible surfactants). Great emphasis is given to the use of natural compounds (such as biopolymers) for the preparation of the immobilization systems, aiming towards applications on sectors such as foods and cosmetics.
Overview of research activities
Our lab has the expertise in formulation of nanodispersions such as microemulsions and nanoemulsions, especially on biocompatible ones, as well as enzyme immobilization and use for the synthesis of high added value products. Main aim of our group is the broadening of their applications in different aspects of life from food products to cosmetics and pharmaceuticals.
Formulation of water-in-oil (W/O) microemulsion [Video]
Protein from Spirulina, a microscopic and filamentous microalgal cyanobacterium with long history of use as safe food, can be used to fight malnutrition since it is a complete protein, owing all essential amino acids. Moreover, a number of valuable constituents has been identified in the cultivation medium, including proteins, exopolysaccharides and lipids.
This project focuses on protein/lipid extraction and isolation from microalgae biomass or cultivation medium.
Proteins can serve as flexible materials for the generation of nanocarriers, as they can be easily subjected to modifications that provide the carrier with additional properties, such as the attenuation of protein corona formation on the carrier surface upon contact with biological fluids, targeting of specific tissues/protein receptors, etc.
The project investigates protein nanocarriers that bind/encapsulate primarily hydrophobic compounds with the purpose of enhancing their bioavailability. For this project, both recombinant and isolated proteins are being used, and nanocarrier formation takes place either by self-assembly or chemical/mechanical processing of the protein.
Microemulsions are regarded as "the ultimate enzyme microreactors" for liquid catalysis. Their structure, composed by water nanodroplets dispersed in a non-polar medium, provides several benefits for their use as media for enzymatic transformations. They have the ability to overcome the solubility limitations of hydrophobic substrates, enhance the enzymatic activity (superactivity phenomenon) and stability, while providing an interface for surface-active enzymes. Of particular interest is the use of such systems to study biotransformations catalyzed by lipases and other enzymes towards the production of high added value products. Weak self-assembly systems consisted by a nonpolar hydrocarbon, an alcohol and water can form macroscopically homogeneous liquid media with distinct microenvironments of varying polarity. These ternary systems are very similar to classic water in oil microemulsions although they don’t have any amphiphilic surfactant. They were thus, initially called “surfactantless” or “detergentless” microemulsions. One of the main application fields that these systems cover is the one of biocatalysis in non-conventional media, a pillar of industrial biotechnology.
Gels, either based on water-in-oil microemulsions (organogels) or on water (hydrogels) can be formed using various natural polymers such as gelatin, agar, chitosan or cellulose derivatives. Enzymes entrapped in such systems can keep their activity and enhance their stability within the gel matrix. The importance of these catalysts leans on their numerous potential biotechnological applications. An important example is the use of various lipase-containing systems for hydrolytic or synthetic reactions.
Nanodispersions based on biocompatible, safe materials are formulated as delivery vehicles of drugs with potential anticancer activity. Most of the chemotherapeutic drugs currently available are poorly water-soluble and need to have their bioavailability improved through the development of enabled formulations and effective delivery mechanisms.
One of the most important fields of modern "translational research" is the design and development of delivery systems as carriers for chemotherapeutic drugs. Their targeted-controlled release in tumor tissues is an attempt to avoid side effects and face major clinical problems such as tumor resistance.
In context of STHENOS project, (O/W) micro- and nanoemulsions were formulated as delivery vehicles of lipophilic antitumor drugs against various cancer types (e.g melanoma) correlated with the BRAFV600E mutation. Structural characterization of the proposed systems was obtained using viscosity, dynamic light scattering (DLS), electron paramagnetic resonance (EPR) spectroscopy and cryogenic-transmission electron microscopy (Cryo-TEM). This project was followed by STHENOS-β which aims to the formulation of biocompatible gels and (O/W) micro- and nanoemulsions as delivery vehicles of lead compounds against cancer types correlated with the BRAFV600E mutation but also lead compounds as inhibitors of glucocorticoid receptor (GR).
The drug delivery through nasal route offers advantages including rapid absorption, avoidance of first-pass hepatic metabolism etc. In this respect, nanodispersions based on Extra Virgin Olive Oil will be used as carriers for a variety of bioactive compounds through nasal route. Intranasal administration of nanodispersions offers a practical, non-invasive, convenient and cost-effective route of administration. The nanodispersions will be tested for their possible toxic in nasal cells. It has to be underlined that preliminary studies have not shown any toxic effect in human colon cells of the above systems even in high concentrations. The construction of a nasal epithelium that is mimicking the physiological will be constructed with the cultivation of specific cell lines in Transwell device. The artificial epithelium will be used in order to prove if the proposed systems enhance the permeability of different encapsulated compounds in nasal mucosa and if cause morphological changes in the epithelium.
Natural sunscreen filters are substances such as antioxidants, proteins etc., that can be found in nature. Cleanway Ltd leans towards the exploitation of the products that can be found in the Greek biodiversity (spirulina and extra virgin olive oil) for the extraction of such bioactive substances. This way, the addition of chemical filters, that have great cost for the human health as well as for the environment, can be reduced and/or avoided entirely. The subject of the present study is the development of nanoemulsion based sunscreens with encapsulated antioxidants, peptides, proteins and/or other plant based bioactive substances, which can subserve the sunscreen effect (UV boosters).
IKY POST DOC SCHOLARSHIPS - PARTNERSHIP AGREEMENT (PA) 2014-2020
Dr. I. Matis was honoured with a scholarship for the project “Directed Formation of the Protein Corona on Bioactive Compound Nanocarriers”. Budget: 26.400 €.
Grantee: Dr. I. Matis. Coordinator Dr. A. Xenakis
Scholarship from Hellenic Foundation and Research Foundation (HFRI) and the General Secretariat for Research and Technology (GSRT)
PhD student E. Mitsou was selected by the Hellenic Foundation and Research Foundation for a 18month PhD scholarship.
Scholarship from Institut Français Grèce
Dr. M. Chatzidaki was selected by the Institut Français Gréce for a three-month Post-Doc scholarship «Séjours scientifiques de haut niveau» 2016.
Innovathens 2016 – Olea team
Our team has been selected by scientists specialized in food science, economists and entrepreneurs to participate in the Business Accelerator called “Food for Business” organized by the Innovathens team- Hub of Innovation and Entrepreneurship, Technopolis city of Athens. (For more information visit: http://www.innovathens.gr/). “Food for Business” is a three-month program of accelerated advisory that provides high quality information services, education and personalized mentoring/coaching in groups and startups who aspire to try the fields of foods, gastronomy and tourism.
Papadimitriou K., Chatzidaki M. D., Alexandraki S., Papadimitriou V., Tsakalidou E., Xenakis A. 2015. “Water-in-oil (W/O) microemulsions as carriers of bacteriocins for the antimicrobial protection of foods” (OBI – 20151008858).
Chatzidaki M. D., Mitsou E., Theochari I., Papadimitriou V., Xenakis A., 2015. “Edible microemulsions with encapsulated plant extracts as dressing type products” (OBI – 20151008863).
Filippou K., Zoumpanioti M., Xenakis A. 2013. “Immobilized catalyst in a continuous flow system for the synthesis of high added value products” (OBI – 20131008307).
Kalaitzaki A., Xenakis A., Papadimitriou V. 2013 “Biocompatible nanodispersions as media for encapsulating bioactive substances with phytoprotective activity” (OBI- 20130100305).
The conference of the European Colloid and Interface Society will be held in Athens organized by our team and a group of prestigious scientists in the field of colloids. The annual ECIS Conference brings together participants from the broad, interdisciplinary field of colloid and interface science, including chemists, physicists, biologists and engineers.
9th Greek Lipid Forum / National Hellenic Research Foundation, Athens, Greece
The aim of the conference, with the participation of our team in the organizing committee, is to provide a platform for the exchange of scientific and technological knowledge in the field of lipids and oils. The aim of GLF (member of the European Federation for the Science and Technology of Lipids) is to inform the public about current lipid developments by bringing together scientists from the academic and research community, who deal with olive oil, edible fats, their technology and applications, non-food lipid applications etc.
Our team has an active participation in Science Communication Projects such as Researcher’s Night and Athens Science Festival. Our aim is to give the opportunity to children to explore scientific and technological advancments in an entertaining way.
DLS is a non-invasive, well-established technique that can be used to determine the size and the size distribution of particles in the sub-micron region. Some of the DLS applications which are used in our lab are the determination of particle size, polydispersity index and z-potential of different types of emulsions such as microemulsions and nanoemulsions.
EPR is a powerful, versatile, nondestructive and nonintrusive analytical method yielding structural and dynamical information, even from ongoing chemical or physical processes.
The Bruker EMX EPR spectometer is a research grade scientific instrument. It is capable of routine measurements, as well as sophisticated and advanced experiments.
Some of the EPR applications which are commonly used in our laboratory are listed below:
Study of interfacial properties and dynamic structures in different nanodispersions, using spin labels such as 5-doxyl stearic acid (5-DSA)
Investigation of proteins dynamics and conformation in a variety of environments by bounding an enzyme’s active site with appropriate spin-label reagents
Sensitive and rapid method (spin trapping) for evaluating the antioxidant activity of potential antioxidant compounds by using free radicals (Galvinoxyl, DPPH etc.)
Detection of paramagnetic species
Our group has expertise in EPR studies with decades of experience and plentiful bibliography (See the complete list publication archive) in a wide range of applications from biotechnology and pharmaceuticals to food applications.
PandaPlus 1000 is a table-top laboratory unit, designed for continuous operation at high pressure, for high pressure processing of nanoparticles, nanodispersions, nanoemulsions and for cell disruption. In addition, it can be used for processing dairy products, fruit juices, liquid food, food additives and ingredients as well as cosmetic and chemical products. Tests can be run even with small sample volumes, and the results are comparable with those achieves on bigger size machines.
It is a homogenizer for general laboratory purpose with electronically stabilized speed control. The stirring medium is subjected to a combination of mechanical shearing and sonic energy. It is suitable for the formulation of emulsions, nanoemulsions and Pickering emulsions
Franz Diffusion Cell is generally regarded as the most common in vitro release testing method. It is often used to monitor release of active compounds from semi-solid drug products across a membrane (synthetic membrane, a tissue construct or biological sample such as cadaver skin). It is suitable for testing the release of active substances encapsulated in microemulsions or gels.
Our high pressure apparatus is suitable for performing enzymatically catalyzed reactions under supercritical conditions. Since supercritical carbon dioxide (scCO2) has emerged as perhaps the most promising “green” solvent (apart from water) due to its being toxicologically harmless, not inflammable, readily available and inexpensive, enzymatic reactions in scCO2 are increasingly attracting interest. Using this apparatus, we aim in replacement of any organic solvent used in the conventional biocatalytic process by harmless CO2.
The hybrid quadrupole / linear ion trap technology of the 3200 QTRAP System offers the ability to identify and quantitate components from complex samples in a single run. LC-MS/MS can be used for qualitative and quantitative analysis of lipid complex mixtures but also for the monitoring of chemical reactions (esterification, epoxidation).
The mass spectrometer has a wide range of applications and among others, it has been used in our lab for the identification and determination of smoking biomarkers in meconium.
Scientific Technical Personnel
Mr. G. Sotiroudis, MSc. Pharmacist
Researcher Emeritus
Dr. Theodoros G. Sotiroudis, Chemist
Postdoctoral Fellows and Scientific Collaborators
Dr. Spyridon Avramiotis, Chemist
Dr. Maria Chatzidaki, Chemical Engineer
Dr. Ilias Matis, Chemist
Dr. Evgenia Mitsou, Biologist
Dr. Ioanna Theochari, Chemist
Elpida Gad, MSc. Chemist
PhD Students
Sotiria Demisli, Msc. Chemist, University of Thessaly, Greece
Konstantina Matskou, MSc Biologist, National and Kapodistrian University of Athens
Evdokia Vassiliadi, Msc. Biologist, University of Ioannina, Greece
Msc. Students
Eleni Galani, MSc Chemist, University of Ioannina, Greece
Panagiota Halkaki, Biologist, University of Thessaly, Greece
Vassilis Makaronis, University of Ioannina, Greece
Bsc. students
Spiridoula Arapi, Agricultural University of Athens
Kalliopi Kourioti, Department of Biological Applications, University of Ioannina, Greece
Vassiliki Kokkovou, Department of Biology National and Kapodistrian University of Athens, Greece
Xrysa Kourtesi, National Technical University of Athens, Greece
Despina Noti, Department of Biological Applications, University of Ioannina, Greece
Maria Vlachou, Department of Biological Applications, University of Ioannina, Greece
The collaboration of our group with industries has three main axes:
Common funded projects
Provision of services in order to determine properties of different types of products (use of EPR, DLS etc.)
Product development through contracts (food products, cosmetics etc.)
Common funded projects
Cleanway Ltd
Nano-UV-Extracts “Application of nanotechnology for the development of sunscreens using natural extracts” NSRF, Operational Program Competitiveness, Entrepreneurship and Innovation 2014-2020.
QUALIA PHARMA
QFytoTera: Nanoemulsions of plant oils with moisturizing and insect repellent properties”. NSRF, Operational Programme Competitiveness, Entrepreneurship and Innovation 2014- 2020
PAGONIS-DAIRY
Development of bioactives containing nanoemulsions as adducts in dairy products
ALGAE S.A.
“Bioconversion of CO2 into high added value bioproducts through sustainable microalgae cultivation processes” NSRF, Operational Programme Competitiveness, Entrepreneurship and Innovation 2014- 2020
JOTIS S.A.
“RIS3-AGROFOOD” “Study and exploitation of olive oil as innovative raw material for special industrial applications to baby food products”.
Public Power Corporation (ΔΕΗ)
“Bioconversion of CO2 into high added value bioproducts through sustainable microalgae cultivation processes” NSRF, Operational Programme Competitiveness, Entrepreneurship and Innovation 2014- 2020
Product development
OXFORD BIOLABS S.A.
Development of improved formulation of a microemulsion containing hair care product
Cirad, Argicultural Research for Development, Montpellier, France
Institut de Chimie & Biologie des Membranes & des Nano-objets (CBMN), Pessac, France
CNRS- Centre National de la Recherche Scientifique, Marseille, France
University of Örebro, Örebro, Sweeden
Technion- Israel Institue of Technology, Haifa, Israel
University of Copenhagen, Copenhagen, Denmark
Ecole Supérieure des Industries Alimentaires de Tunis, Tunis, Tunisia
Ege University, Izmir, Turkey
National Engineering School in Sfax, Sfax, Tunisia
Department of Pharmaceutical Technology and Cosmetology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
Department of Chemistry, Univercity of Nicosia, Cyprus
Faculty of Pharmacy, Federal University of Rio de Janeiro, Brazil
Biocatalysis and Organic Synthesis Group, Chemistry Institute, Federal University of Rio de Janeiro, Brazil
State University of Maringa, Department of Chemical Engineering, Maringa, Brazil
Argicultural University of Athens, Athens, Greece
Department of Biological Applications & Biotechnology, University of Ioannina, Ioannina, Greece
Faculty of Biology, National & Kapodistrian University of Athens, Athens, Greece
University of Thessaly, Larissa, Greece
Department of Food Technology, Technological Educational Institute of Athens, Athens, Greece
Department of Food Technology, School of Argicultural Technology, Thessaloniki, Greece
Benaki Phytopathological Institute, Kifissia, Greece
Material Synthesis and Physical Chemistry, Theoretical & Physical Chemistry Institute, National Hellenic Research Foundation, Athens, Greece
Funded projects
National Strategic Reference Framework Program, “Application of nanotechnology for the development of sunscreens using natural extracts” (Nano-UV-Extracts). Operational Program Competitiveness, Entrepreneurship and Innovation 2014-2020 (EPAnEK) Budget: 425.830 € (Budget ICB/NHRF 100.000 €). Coordinator Dr. A.Xenakis
National Strategic Reference Framework Program, “QFytoTera: Nanoemulsions of plant oils with moisturizing and insect repellent properties”. NSRF, Operational Programme Competitiveness, Entrepreneurship and Innovation 2014- 2020, Industrial Partner: Qualia Pharma (Coordinator Dr S. Zographos, IBMCB/NHRF) (Budget: 630,005.37 €, Budget IBMCB/NHRF: 289.900) WP1 leader Dr. V. Papadimitriou
National Strategic Reference Framework Program “Bioconversion of CO2 into high added value bioproducts through sustainable microalgae cultivation processes”. NSRF, Operational Programme Competitiveness, Entrepreneurship and Innovation 2014-2020 and co-financed by Greece and the EU (European Regional Development Fund), Industrial partners Algae S.A. and Public Power Corporation (ΔΕΗ). Budget 1.000.000 € (Biomimetics & Nanobiotechnology 150.000,10 €), Coordinator CERTH, PI for NHRF: Dr. Maria Zoumpanioti
National Strategic Reference Framework Program- “RIS3-AGROFOOD”. “Study and exploitation of olive oil as innovative raw material for special industrial applications to baby food products”. Operational Programme “West Greece 2014-2020”. Industrial partner JOTIS S.A. Budget 250.000 € (Biomimetics & Nanobiotechnology 65.000 €), Coordinator JOTIS, PI for NHRF: Dr. Maria Zoumpanioti
National Strategic Reference Framework Program, Action STHENOS-β/KRIPIS “Targeted therapeutic approaches against degenerative diseases with special focus on cancer and ageing-optimisation of the targeted bioactive molecules” (NSRF 2015–2020). Budget 790.000,00 € (Biomimetics 84,968€). Coordinator Dr A. Pintzas
“Nanobiotechnology”, National Hellenic Research Foundation Project 2402, 2015-2018, Budget 162.635 €. Coordinator NHRF: Dr. A. Xenakis.
G. Vaz, A. Clementino, E. Mitsou, E. Ferrari, F. B., C. Sissa, A. Xenakis, F. Sonvico, C. Dora ”In vitro evaluation of curcumin and quercetin loaded nanoemulsions for intranasal administration: effect of surface charge and viscosity” Pharmaceutics, (2022) 14, 194 doi.org/10.3390/pharmaceutics14010194
E. Mitsou, I. Theochari, E. Vassiliadi, F. Nallet, A. Xenakis, ”Short-Wave and Near Infrared π-Conjugated Polymers hosted in a Biocompatible Microemulsion: A Pioneer Approach for Photoacoustic Contrast Agents” J.Mater.Chem.B (2022) https://doi.org/10.1039/D1TB02257A
S. Demisli, M.D. Chatzidaki, A. Xenakis, V. Papadimitriou ”Recent progress on nano-carriers fabrication for food applications with special reference to olive oil-based systems” Curr.Opin.Food Sci. (2022) 43, 146-154, doi.org/10.1016/j.cofs.2021.11.012
I. Theochari, T. Ilic, I. Nicolic, V. Dobricic, A.Tenchiou, D. Papahatjis, S. Savic, A. Xenakis, V. Papadimitriou, V. Pletsa, Evaluation of Oil-In-Water Microemulsions as carriers of lipophilic, cytotoxic compounds for dermal applications Biomimetics, 2021, 6, 10. doi:10.3390/biomimetics6010010
Gligorijević, N.; Minić, S.; Radibratović, M.; Papadimitriou, V.; Nedić, O.; Sotiroudis, T.G.; Nikolić, M.R. Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2012, 251, 119483. doi.org/10.1016/j.saa.2021.119483
I. Theochari, A. Giatropoulos, V. Papadimitriou, V. Karras, G. Balatsos, D. Papachristos, A Michaelakis. “Physicochemical Characteristics of Four Limonene-Based Nanoemulsions and Their Larvicidal Properties against Two Mosquito Species, Aedes albopictus and Culex pipiens molestus”. Insects, 2020, 11(11), 740. doi.org/10.3390/insects11110740
M. Chatzidaki, I. Kostopoulou, C. Kourtesi, I. Pitterou, S. Avramiotis, A. Xenakis, A. Detsi. “β-Cyclodextrin as carrier of novel antioxidants: A structural and efficacy study”. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2020, 603, 125262. doi.org/10.1016/j.colsurfa.2020.125262
S. Demisli, I. Theochari, P. Christodoulou, M. Zervou, A. Xenakis, V. Papadimitriou. “Structure, activity and dynamics of extra virgin olive oil-in-water nanoemulsions loaded with vitamin D3 and calcium citrate.” Journal of Molecular Liquids, 2020, 112908 DOI: 10.1016/j.molliq.2020.112908
S. Demisli, E. Mitsou, V. Pletsa, A. Xenakis, V. Papadimitriou. “Development and Study of Nanoemulsions and Nanoemulsion-Based Hydrogels for the Encapsulation of Lipophilic Compounds”. Nanomaterials,2020, 10(12), 2464 doi.org/10.3390/nano10122464
E. Vassiliadi, E. Mitsou, S. Avramiotis, C.L. Chochos, F. Pirolt, M. Medebach, O. Glatter, M. Zoumpanioti. “Structural Study of (Hydroxypropyl) Methyl Cellulose Microemulsion-Based Gels Used for Biocompatible Encapsulations”. Nanomaterials, 2020 10(11), 2204, doi.org/10.3390/nano10112204
E. Mitsou, V. Pletsa, G.T. Sotiroudis, P. Panine, M. Zoumpanioti, A. Xenakis. “Development of a microemulsion for encapsulation and delivery of gallic acid. The role of chitosan”. Colloids and Surfaces B: Biointerfaces,2020, 190, 110974, doi.org/10.1016/j.colsurfb.2020.110974
I. Nikolic, E. Mitsou, I. Pantelic, D. Randjelovic, B. Markovic, V. Papadimitriou, A. Xenakis, D.J. Jasmin Lunter, A. Zugic, S. Savic. “Microstructure and biopharmaceutical performances of curcumin-loaded low-energy nanoemulsions containing eucalyptol and pinene: Terpenes’ role overcome penetration enhancement effect?” European Journal of Pharmaceutical Sciences, 2020, 142, 105135, doi.org/10.1016/j.ejps.2019.105135
I. Nikolić, E. Mitsou, A. Damjanović, V Papadimitriou, J. Antić-Stanković, B. Stanojević, A. Xenakis, S. Savić. “Curcumin-loaded low-energy nanoemulsions: Linking EPR spectroscopy-analysed microstructure and antioxidant potential with in vitro evaluated biological activity.” Journal of Molecular Liquids, 2020, 301, DOI: 10.1016/j.molliq.2020.112479
E. Mitsou, A. Dupin, A. H. Sassi, J. Monteil, G.T. Sotiroudis, F. Leal-Calderon, A. Xenakis. “Hydroxytyrosol encapsulated in biocompatible water-in-oil microemulsions: How the structure affects in vitro absorption.” Colloids and Surfaces B: Biointerfaces, 2019, 184, 110482, doi.org/10.1016/j.colsurfb.2019.110482
E. Mitsou, E. P. Kalogianni, D. Georgiou, H. Stamatis, A. Xenakis, M. Zoumpanioti. “Formulation and structural study of a biocompatible water-in-oil microemulsion as an appropriate enzyme carrier: The model case of Horseradish peroxidase”. Langmuir, 2019, 35, 150-160, doi.org/10.1021/acs.langmuir.8b03124
P. C Ioannou, C. Arbez-Gindre,M. Zoumpanioti, C.P. Raptopoulou,V. Psycharis,I.D. Kostas, P. Kyritsis. “Catalytic reactivity of the complexes [Pd {(Ph2P) 2N (tBu)-P, P´} X2], X= Cl, Br, I, in the Suzuki-Miyaura C− C coupling reaction: Probing effects of the halogeno ligand X− and the ligand's tBu group”. Journal of Organometallic Chemistry, 2019, 879, 40-46 doi.org/10.1016/j.jorganchem.2018.10.006Ioannou, P. C., Arbez-Gindre, C., Zoumpanioti, M., Raptopoulou, C.P., Psycharis, V., Kostas, I.D., Kyritsis, P. Catalytic reactivity of the complexes [Pd{(Ph2P)2N(tBu)-P,P´}X2], X = Cl, Br, I, in the Suzuki-Miyaura C−C coupling reaction: Probing effects of the halogeno ligand X− and the ligand's tBu group. J. Organomet. Chem. (2019) 879, 40-46. (IF 1.946)
Kavga, A., Strati, I.F., Sinanoglou, V.J., Fotakis, C., Sotiroudis, G., Christodoulou, P., Zoumpoulakis, P. Evaluating the experimental cultivation of peppers in low-energy-demand greenhouses. An interdisciplinary study. J Sci Food Agr (2019) 99 (2), 781-789 (IF 2.379)
Chatzidaki, M.D, Balkiza, F., Gad, E., Alexandraki, V., Avramiotis, S., Georgalaki, M., Papadimitriou, V., Tsakalidou, E., Papadimitriou, K., Xenakis, A. Reverse micelles as nano-carriers of nisin against foodborne pathogens. Part II: The case of essential oils, Food Chem., (2019) 278, 415-423. (IF 4.946)
Chatzidaki, M.D, Xenakis A, Food Soft Nano-Dispersions for Bioactive Delivery: General Concepts and Applications Encyclopedia of Food Chemistry (2019) 2, 701-707
Vassiliadi E., Xenakis, A. Zoumpanioti, M.. Chitosan hydrogels: A new and simple matrix for lipase catalysed biosynthesis. Mol Catal, (2018) 45, 206-212
Golfomitsou, I., Mitsou, E., Xenakis, A., Papadimitriou, V. Development of O/W nanoemulsions as carriers of vitamin D: A structural study, J. Mol. Liq. (2018) 268, 734–742. (IF 4.513)
Chatzidaki, M.D.; Papadimitriou, K.; Alexandraki, V.; Balkiza, F.; Georgalaki, M.; Papadimitriou, V.; Tsakalidou, E.;Xenakis, A. Reverse Micelles as Nanocarriers of Nisin against Foodborne Pathogens. Food Chem. (2018), 255, 97–103. (IF 4.946)
Theochari, I.;Papadimitriou, V.; Papahatjis, D.; Assimomytis, N.; Pappou, E.; Pratsinis, H.; Xenakis, A.; Vasiliki Pletsa, V. Oil-in-water microemulsions as hosts for benzothiophene cytotoxic compounds. An effective combination. Biomimetics (2018) 3, 13.
Chaari, M., Theochari, I., Papadimitriou, V.,Xenakis, A., Ammar, E. Encapsulation of carotenoids extracted from halophilic Archaea in oil-in-water (O/W) micro- and nano-emulsions. Colloids and Surfaces B (2018) 161, 219-227 (IF 3.902)
Bubic Pajić, N.; Nikolic, I.; Mitsou, E.;Papadimitriou, V.;Xenakis, A.; Randjelovic, D.; Dobric, V.; Smitran, A.; Cekic, N.; Calija, B.; Savic, S. Biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate: phase behavior study and microstructure influence on drug biopharamaceutical properties. Journal of Molecular Liquids (2018) 272, 746-758 (IF 4.513)
