INSTITUTE OF CHEMICAL BIOLOGY
 
  Drug Discovery
  Molecular Analysis
  Organic and Organometallic Chemistry
  Medicinal Chemistry
  Synthetic Medicinal Chemistry and Chemical Biology
  Identification & validation of novel therapeutic targets - Biological evaluation of bioactive small molecules and drugs
  Structural Biology & Chemistry
  Molecular Endocrinology
  Signal Mediated Gene Expression
  Molecular & Cellular Ageing
  Biomedical Applications
  Holistic Approaches in Health
  Environment and Health
  Metabolic Engineering-Bioinformatics
  Biomarker Discovery & Translational Research
  Biotechnology
  Enzyme and Synthetic Biotechnology
  Biomimetics & Nanobiotechnology
  Conjugated Polymers for Healthcare, Bioelectronics and Bioimaging

 

Structural Biology & Chemistry
Dr. Evangelia D. Chrysina | Senior Researcher, Group Leader

 

Chrysina Group specializes on Structural Biology with emphasis on Biomedical and Biotechnological applications. The Group performs functional and structural studies of proteins of pharmaceutical and biotechnological interest in the thematic areas of Life Sciences, Bioinformatics, Natural Sciences, Chemical Engineering and Technology.

Mission

The Chrysina group mission is to demystify the 3D protein space by Integrative Structural Biology and Structural Bioinformatics for Biomedical and Biotechnological Applications.

Vision

The vision of the group is to address pressing societal challenges by applying integrated approaches.

Chrysina group passion for Structural Biology coupled with the engineering background of the PI is the driving force to continuously explore, enrich and push boundaries in the aims set. With the aim to address pressing societal challenges by applying integrated approaches, Structural Biology has unveiled the need for and the dynamic of intersectoral cooperation between biologists, chemists, engineers and computer scientists. It has significantly contributed to understanding protein function at cellular level, supported by the improvements in the infrastructures. Within this field one can witness the power of genuine integration of disciplines with the aim of delivering solutions. Thus, the full breadth of biological processes may be captured, encompassing the full chain, from data, to structure, to function, converting information to knowledge. To keep up with the rapid advances in this field, structural biology now gives emphasis on systems and has developed a cross-talk with molecular, cellular and chemical biology, as well as biophysics and metabolic engineering to shed light on protein structure-function relationships that dictate and regulate the metabolic processes.

 

Targets and strategic plan for the future

The Chrysina group at the Institute of Chemical Biology (ICB), NHRF envisages to be a point of reference for Integrative Structural Biology in South-east Europe and the Mediterranean area welcoming young scientists, collaborators and users of Instruct-EL hub, who wish to explore the “three-dimensional space”, whether they have prior knowledge in the field of structural biology or not. Instruct-EL hub, is one of the core nodes of the national distributed research infrastructure on structural biology Inspired-RIs, which is coordinated by ED Chrysina and NHRF.

Diagram  Description automatically generated More information on Inspired-RIs and the services provided are available at https://www.inspired-ris.gr, https://inspired-ris.catalogue.athenarc.gr

Core Values

The core values to achieve Chrysina group vision are: Excellence, a founding value that has allowed the Group to achieve its goals until present, building a strong collaborative international network, increasing awareness of the Group on disruptive and groundbreaking research in Europe and worldwide and bringing it at the forefront of a new research and innovation ecosystem. Free circulation of ideas and teamwork to sustain excellence and the leading role of the Group, building on the experience gained; equitable treatment to all group members, an essential component, thus securing the trust that has been built across the members; transparency in all rules and procedures, including recruitment and career development based on merit, professionalism, gender equality and diversity & inclusion; effectiveness in implementing the ICB-NHRF decisions; continuous improvement in promoting the Group and ICB-NHRF to the wider community worldwide, by raising funds to achieve the objectives set and encouraging the professional development of key members to meet the emerging needs; they may benefit from different mobility and transnational access schemes either as visiting scientist in renown structural biology labs, or as users of Large European RIs. To capitalize on the existing and new competitive advantages of Chrysina Group, and fulfil the objectives set, driven by the aforementioned core values, four strategic pillars are selected: Group members/users (are encouraged to get trained and develop skills in the latest advances of structural biology and benefit from available transnational access schemes to RIs); synergies (capacity building providing incentives to groups/private sector from Balkans and SE-Europe), operational structure (create task forces to approach users, build capacity in technology transfer) and finances (coordinate and/or participate in “Horizon Europe” funding opportunities; seek for additional funding sources (e.g. from patients or disease associations)). Dr Chrysina remains committed to her cause: to bring structural biology at the forefront of research and help all people recognize the role it plays in understanding the mechanisms of life.

 

Keywords

Structural Biology, Biomedicine, Biotechnology, Biochemistry/Enzymes, Biophysics, Biocatalysts, Structural genomics-proteomics, Structure-based drug design, X-ray crystallography, Type 2 Diabetes, Structural Bioinformatics, Structure-Function Relationships, Mechanisms of enzymic action, Biochemical Engineering, Process development, Development of New Methods and Processes

Scientific Directions

The Chrysina Group includes four distinct yet interconnected pillars as main scientific directions:

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Main scientific directions

Structural Biology

Structural Biology studies currently are performed by X-ray protein crystallography and in the future by other techniques such as SAXS and cryo-EM focusing on:

a. enzymes directed implicated in glycogen metabolism for the development of new antidiabetic agents using the structure-based drug design approach.
b. biomass degrading enzymes

 

Enzymes directed implicated in glycogen metabolism for the development of new antidiabetic agents using the structure-based drug design approach

This approach has been applied on glycogen phosphorylase (GP), the first enzyme for which control by reversible phosphorylation was established, that catalyses the glycogen degradation to glucose-1-phosphate, which is then released as glucose in the bloodstream. GP is validated target for treating type 2 diabetes. The long-standing collaboration with synthetic chemists in Greece and abroad has resulted in the investigation of >300 compounds that have been examined by kinetic and structural studies using X-ray protein crystallography.

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The structure-based drug design approach as presented by Chegkazi, M.S., Mamais, M., Sotiropoulou, A.I., Chrysina, E.D. (2018). Rational Drug Design Using Integrative Structural Biology. Methods Mol. Biol. 1824, 89-111, doi: 10.1007/978-1-4939-8630-9_6

A fruitful cooperation with Gimisis group, Dept. of Chemistry at NKUA, has resulted in GP inhibitors in the low nM range, some of which have fluorescent properties and may be further used for cell-based assays. Indeed, our paper discusses the binding mode of a glucose-based molecular rotor, inhibitor of GP, as a probe of cellular enzymatic function.

image image

Mamais, M., Esposti, A.D. Kouloumoundra, V., Gustavsson, T., Monti, F., Venturini, A., *Chrysina, E.D., Markovitsi, D., Gimisis, T. (2017). A New Potent Inhibitor of Glycogen Phosphorylase Reveals the Basicity of the Catalytic Site. Chemistry- A European Journal 23, 8800-8805 (IF2017 5.16) (Frontipiece)
PDB ID
: 5MEM

Minadakis, M-P., Mavreas, K.F., Neofytos, D.D., Paschou, M., Kogkaki A., Athanasiou, V., Mamais, M., Veclani, D., Iatrou, H., Venturini, A., *Chrysina, E.D., Papazafiri, P., Gimisis, T. (2022) A glucose-based molecular rotor inhibitor of glycogen phosphorylase as a probe of cellular enzymatic function. Org. Biomol. Chem. 20, 2407-2423 (IF2021-22 3.876) (Front Cover)
PDB ID: 7Q5I

 

Biomass degrading enzymes

Biomass degrading enzymes of biotechnological interest are of key importance for industrial processes. Our work so far involves biochemical and structural characterization of hemicellulases (xylanases, glucuronoyl and feruloyl esterases) using European large-scale facilities such as SRS at PETRA III/DESY, EMBL-Hamburg Unit. With the aim to explain the thermal stability of these enzymes their 3D structure has been determined at high resolution. Selected published work includes the 3D crystal structures of a novel glucuronoyl esterase from S. thermophile and a new feruloyl esterase from F. oxysporum (in collab. with Christakopoulos and Topakas groups, School of Chemical Engineering, NTUA). Dr Chrysina also has an ongoing collaboration with Vorgias and Hatzinikolaou groups, Dept. of Biology, NKUA on more biocatalysts of this type as well as Skretas group, ICB-NHRF.

image

The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family as presented by Dimarogona, M., Topakas, E., Christakopoulos, P. & ›Chrysina, E. D. (2020) FEBS Lett. 1873-3468.13776 (IF2020 6.750) PDB ID: 6FAT

Structural Bioinformatics / Integrative Structural Biology

Structural Biology has unveiled the need for and the dynamic of intersectoral cooperation between biologists, chemists, engineers and computer scientists has become unveiled. Within this field one can witness the power of genuine integration of disciplines with the aim of delivering solutions such as new drugs, vaccines, etc. Thus, the full breadth of biological processes may be captured, encompassing the full chain, from data, to structure, to function, converting information to knowledge.

Chrysina group pursues this aim by

  • identifying structural determinants that dictate ligand binding and enzymic function employing geometric algorithms and structural bioinformatics (in collab. with Emiris group, Dept. of Informatics/NTUA);
  • reconstructing the metabolic network that connects these proteins with emphasis on their interactions (in collab. with Klapa group, FORTH-ICEHT)
  • fully characterize the proteins of interest by biophysical methods and investigate protein:polymer interactions for potential biotechnological applications (in collab. with Pispas group, TPCI-NHRF.
New methods and process development

Chrysina group works on the design of new methods and process development with emphasis on:

  • Protein crystallization
  • Proteins as Biomaterials, in collaboration with experts in Biopolymers
  • Computational Mechanics for advanced simulations

 

Group Structure and Personnel

Dr. Evangelia D. Chrysina
Chemical Engineer, PhD in Biochemistry, Docent at Orebro University, Senior Researcher at ICB-NHRF

Role within the Institute and the NHRF

  • Group leader/Senior Researcher at ICB-NHRF (since 2016)
  • Coordinator of Inspired-RIs (MIS 5002550), the distributed National Research Infrastructures on Structural Biology, which is affiliated with the ESFRI Landmark Instruct-ERIC
  • Scientist in charge at NHRF for representing Greece in the «Integrated Structural Biology Infrastructure – Instruct», during the preparatory and operational phase of Instruct ESFRI project (since 2015); member of Instruct-ERIC Council as observer, being the authorized representative of Greece for NHRF and GSRT (since 2017)
  • Chair of NHRF's Open Data Committee

 

Current Group members

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Anastasia Sotiropoulou, Chemist, MSc in Biochemistry
PhD Student, Department of Biology, National and Kapodistrian University of Athens (funded by State Scholarship Foundation IKY) (2016 - present) (co-advisor, in collab. with Assoc. Prof. D. Hatzinikolaou, main advisor).
Research activity: structural studies of enzymes of biotechnological interest-degradation of plant biomass by β-hydrolases.

 

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Pandora Karakousi, Biochemist-Biotechnologist, MSc in Microbial Biotechnology
PhD Student, Department of Chemistry, National and Kapodistrian University of Athens (funded by H2020-INFRADEV No. 731005: Instruct-ULTRA) (2019 - present) (main advisor)
Research activity: structural studies of enzymes implicated in glycogen metabolism (PhD thesis) and intrinsically disordered proteins in cooperation with Uni-Pharma S.A. (a side project)

 

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Ioanna Gerogianni, Chemist, MSc in Medicinal Chemistry: Design and Development of Pharmaceutical Products
PhD Student, Department of Chemistry, National and Kapodistrian University of Athens (2019 - present) (main advisor, in collab. with Prof. Th. Mavromoustakos)
Research activity: structural studies of lipoxygenases using X-ray protein crystallography.

 

Nikolaos Nikolopoulos, Mathematician
Post-graduate student, Master’s programme “Algorithms, Logic and Discrete Mathematics”, Department of Informatics and Telecommunications/ Mathematics National and Kapodistrian University of Athens (2020-present) (main advisor, in collab. with Dr M. Klapa, ICEHT-FORTH & Prof. I. Emiris NKUA/ «Athena»)
Research activity: Reconstruction and comparative studies of glycogen metabolism biomolecular networks in different species (MSc thesis)

 

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Konstantina Mpakou, Chemist
Post-graduate student, Master’s programme “Organic Chemistry”, Department of Chemistry, National and Kapodistrian University of Athens (2021-present) (main advisor, in collab. With Prof. T. Gimisis NKUA.
Research activity: Kinetic and structural studies of glycogen phosphorylase

 

Vasiliki Lagouri, Chemist, MSc and PhD in Food Chemistry, Aristotle University of Thessaloniki.
Project Manager (2017-present) (funded by Instruct-EL hub/INSPIRED MIS 5002550) (main advisor)

 

 

Group members (2008 – present)

Since 2008, a total of 61 research scientists have been under ED Chrysina’s supervision, coming from interdisciplinary research fields.

  • seven (7) post-doctoral research scientists
  • four (4) research associates / research technicians
  • two (2) administrative personnnel
  • seven (7) PhD students
  • five (5) PhD students as member of the their supervising committee
  • three (3) PhD students as member of their evaluation committee
  • twenty two (22) postgraduate students, in two (2) of which as member of the examination committee
  • six (6) undergraduate students / Final Year project students
  • five (5) undergraduate students / Research trainees

 

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Past Group members

Post-doctoral Researchers

  • Maria-Despoina Charavgi, Chemist, MSc in Biochemistry, PhD in Chemical Engineering (funded by H2020-INFRADEV No. 731005: Instruct-ULTRA; Instruct-EL hub/INSPIRED MIS 5002550; States Scholarship Foundation) (2018-2021) (main advisor)
  • Maria Karayianni, Physicist, funded by Instruct-EL hub/INSPIRED MIS 5002550 (2020-2021) (joint advisor with Dr A. Pispas, TPCI-NHRF);
  • Aristeidis. Papagiannopoulos, Physicist (funded by H2020-INFRADEV No. 731005: Instruct-ULTRA) (2019) (joint advisor with Dr A. Pispas, TPCI-NHRF)
  • Aikaterini Tsitsanou, Chemist, PhD in Biology (funded KRIPIS-II/GSRI:STHENOS B', NHRF) (2018 - 2019) (joint-advisor with Dr S. Zographos, ICB-NHRF);
  • Foteini Andreadaki, Biologist, PhD in Biology (funded by FP7-REGPOT-2009-1-245866: ARCADE) (2010-2012) (main advisor)
  • Iliada Lazoura, BSc, PhD in Biochemistry and Molecular Biology (funded by Matching Funds awarded by GSRI for attracting European funding) (main advisor)
  • Joseph Hayes, Chemist, PhD in Computational Chemistry (funded by Transfer of Knowledge (ToK Marie Curie Program, MTKD-CT-2006-042776) (2008-2011) (main advisor)

 

PhD students

  • Paris Christodoulou, Chemist, MSc in Polymer Chemistry (2021) (funded by Instruct-EL hub/INSPIRED MIS 5002550, joint-advisor with Dr M. Zervou ICB-NHRF)
  • Maria-Despoina Charavgi, Chemist, MSc in Biochemistry, PhD in Chemical Engineering, NTUA (2011-2015) (joint advisor with Prof. Paul Christakopoulos/main advisor) (funded by the programme HERAKLEITOS II)
  • Michail Mamais, Chemist, MSc holder in Organic Chemistry, PhD in Organic Chemistry, NKUA (2011-2015) (joint advisor with Prof. T. Gimisis/main advisor) (funded by the programme HERAKLEITOS II)
  • Maria Dimarogona, Chemical Engineer, PhD in Chemical Engineering, School of Chemical Engineering, NTUA (2007-2012) (joint advisor with Prof. Paul Christakopoulos/main advisor)

 

Post-graduate students

  • Maria Papagiannakou, Diploma in Biological Applications and Technologies, MSc in Cosmetology, Dermapharmacology, Department of Pharmacy, NKUA (2020-2022) (joint advisory with Prof. Michail Rallis)
  • Dionysios D. Neofytos, Chemist, MSc student in “Polymers Science & their application in Industry”, Dept. of Chemistry, NKUA (2019-2021) (co-advisor, collab.: Dr S. Pispas, main advisor);
  • Ioanna Gerogianni, Chemist, MSc: “Medicinal Chemistry: Design & Development of Pharmaceutical Products” Dept. of Chemistry, Univ. of Patras (2018 - 2019) (co-advisor, collab.: Assoc. Prof. Th. Tselios)
  • Pandora Karakousi, Biochemist-Biotechnologist, Post-grad Student, in the MSC: “Microbial Biotechnology” Dept. of Biology, NKUA (2017 - 2018) (co-advisor, collab.: Assoc. Prof. D. Hatzinikolaou)
  • Aikaterini Dalamara, Informatician, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2015 - 2016) (main advisor in collab. with Prof. I. Emiris)
  • Maria Karamolegou, Biologist, MSc in Biochemistry, Department of Chemistry, NKUA (2014-2015) (joint advisor with Prof. K. Galanopoulou)
  • Anastasia Sotiropoulou, Chemist, MSc in Biochemistry, Department of Chemistry, NKUA (2014-2015) (joint advisor with Prof. Ath. Siafaka-Kapadai)
  • Anaxagoras Fotopoulos, Informatician, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2014 - 2015) (joint advisor with Prof. I. Emiris)
  • Athanasios Papathanasiou, Informatician, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2014 - 2015) (joint advisor with Prof. I. Emiris)
  • Katerina Ioannidou, Chemist, MSc in Organic Chemistry, Department of Chemistry, NKUA (2013-2014) (joint advisor with Prof. G. Kokotos)
  • Maria Papakonstantinou, Chemist, MSc in Biochemistry, Department of Chemistry, NKUA (2013-2014) (joint advisor with Prof. Ath. Siafaka-Kapadai)
  • Marios Fytros, Software Engineer, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2013 - 2014) (joint advisor with Prof. I. Emiris)
  • Theodora Manousidou, Informatician, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2011 - 2012) (joint advisor with Prof. I. Emiris)
  • Dionysis Markopoulos, Physicist, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2011 - 2012) (joint advisor with Prof. E. Manolakos)
  • Aikaterini Chatzistamatiou, Chemist, MSc in Biochemistry, Department of Chemistry, NKUA (2009-2011) (joint advisor with Prof. Ath. Siafaka-Kapadai)
  • Magda Chegkazi, Chemist, Chemist, MSc in Biochemistry, Department of Chemistry, NKUA (2009-2011) (joint advisor with Prof. T. Gimisis)
  • Evdoxia Mastroleon, Informatician, MSc in Information Technologies in Medicine and Biology, Department of Informatics and Telecommunications, NKUA (2010 - 2011) (joint advisor with Prof. E. Manolakos)
  • Nikos Mavrokefalos, Chemical Engineer, MSc in Pharmaceutical Chemistry, Department of Pharmacy, NKUA (2010-2011) (joint advisor with Prof. Em. Mikros)
  • Christoforos Zarkadas, Informatician, MSc in Theoretical Informatics, Department of Informatics and Telecommunications, NKUA (2009 - 20121) (joint advisor with Prof. I. Emiris)
  • Paraskevi Skourti, Chemist, MSc in Chemist, MSc in Biochemistry, Department of Chemistry, NKUA (2008-2010) (joint advisor with Prof. Ath. Siafaka-Kapadai)

 

Undergraduate students - Final Year Project students

  • Constantina Vasilakou, Final year Chem. Engineer, School of Chem. Engineering, NTUA (2021) (co-advisor in collab. with Prof. A. Detsi)
  • Constantina Roka, Informatician, Department of Informatics and Telecommunications, NKUA (2020-2021) (joint advisor with Prof. I. Emiris)
  • Dionysios D. Neofytos, Chemist, MSc in Polymer Science, Department of Chemistry, NKUA (2018-2019) (main advisor in collaboration with Prof. Th. Mavromoustakos)
  • Dimitris Athanasiou, Chemical Engineer, School of Chemical Engineering, NTUA (2013) (joint advisor with Assist. Prof. Ev. Topakas)
  • Polina Giannelou, Biologist, Department of Biology NKUA (2012) (joint advisor with Prof. S. Hamodrakas and Assist. Prof. V. Iconomidou)
  • Panagiotis Adam, Biologist, Department of Biology NKUA, (2011) (joint advisor with Prof. K.E. Vorgias)

 

Undergraduate students - Research Trainees

  • Stavroula Kaili, Department of Biology, NKUA (2019) (main advisor)
  • Ifigenia-Ioanna Ioannidis, Chemist, Department of Chemistry, University of Crete (Summer 2019) (main advisor)
  • Ioannis Panetas, Biologist, Department of Biology, Aristotle University of Thessaloniki (2013) (main advisor, in collab. with Assist. Prof. A. Drouzas)
  • Theodoros Spathas, Chemical Engineer, School of Chemical Engineering, NTUA (2011) (main advisor)
  • Stamatis Giannoukos, Chemical Engineer, School of Chemical Engineering, NTUA (2008) (main advisor)

 

Other team members

  • Dimitra Pelekanou, Graphics Designer, (2021) (funded by Instruct-EL hub/INSPIRED MIS 5002550) (main advisor)

 

 

Collaborations

International Scientific Collaborations

Collaborations with academic and research organizations in Greece

Research organizations

  • National Hellenic Research Foundation (NHRF)
  • National Centre for Scientific Research (NCSRD) - «Demokritos»
  • Foundation for Technology and Research (FORTH)
  • «ATHENA» Research Centre
  • Hellenic Pasteur Institute

Academic organizations

  • National & Kapodistrian University of Athens (NKUA)
  • National Technical University of Athens (NTUA)
  • University of Patras
  • University of Thessaly
  • University of Ioannina
  • University of Crete
  • “Demokritus” University of Thrace
  • Aristotle University of Thessaloniki

 

Collaborations with academic and research organizations abroad

  • ΝΚΙ, Amsterdam, The Netherlands
  • European Molecular Biology Laboratory- Hamburg, Germany
  • CNRS- Centre Nationnal de la Recherche Scientifique, IRAMIS, LIDYL, France
  • University of Manchester, UK
  • University of Debrecen, Hungary
  • Université Claude-Bernard Lyon 1, France
  • University of Oxford, UK

 

 

Funding

Research Funding (2008 – present)

  • iNEXT-Discovery, Structural biology for translational research and discovery project no. 871037, Horizon 2020 program of the EC (coordinator: Prof. A. Perrakis, PI for NHRF ED Chrysina, Total Budget: €9,987,756.5, Budget for NHRF: €18,000) (2021-2024) (https://inext-discovery.eu)
  • INSPIRED-The National Research Infrastructures on Integrated Structural Biology, Drug Screening Efforts and Drug target functional characterization” (MIS 5002550). Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme "Competitiveness, Entrepreneurship & Innovation" (National Strategic Reference Framework-NSRF 2014-2020) & co-financed by Greece & the EU (Coordinator: Dr ED Chrysina, Total budget €3,818,820.00, Budget for Instruct-EL hub/NHRF: €540,000.85) (3/9/2018-30/09/2022) (https://www.inspired-ris.gr)
  • Structural optimization of thermophilic multienzymic nanostructures with applications biorefineries (contract no.: 2019-050-0503-18289) (Funding for a Post-doctoral researcher, M-D. Charavgi, MSc, PhD in colalboration with Dr D. Hatzinikolaou, Univ. of Athens, State Scholarships Foundation, total budget: €26,400.00 (01.02.2020 – 31.01.2022)
  • Structural studies of enzymes of biotechnological interest with emphasis on β-hydrolases implicated in plant biomass degradation (contract no.: 2018-050-0502-15915) (Funding for a PhD student, A. Sotiropoulou, MSc, under the joint supervision of Dr D. Hatzinikolaou, Univ. of Athens and Dr E.D. Chrysina, IBMCB-NHRF, State Scholarships Foundation, total budget: €29,408.4 (24.04.2018 – 23.04.2021)
  • Releasing the full potential of Instruct to expand and consolidate infrastructure services for integrated structural life science research: Instruct-ULTRA”. Proposal No. 731005, Horizon2020-INFRADEV-2016-2017 (Development and long-term sustainability of new pan-European research infrastructures, Coordination and support action INFRADEV-03-2016-2017) (Coordinator: Prof. Dave Stuart/Prof. Ray Owens, PI for Greece: Dr ED Chrysina, Total Budget: €395,000, Budget for Instruct-EL/NHRF: €100,000) (https://instruct-ultra.eu/network/instruct-ultra/home)
  • Targeted therapeutic approaches against degenerative diseases, with emphasis on cancer and aging-STHENOS II’’ (01.11/2017-30.11.2020) National Strategic Reference Framework-NSRF, Greek General Secretariat for R&T (GSRT) call ‘KRIPIS’ (Coordinator: Dr Α. Pintzas, Director of IBMCB/NHRF €1,500,000.00, ED Chrysina budget: €26,714.00)
  • «Instruct-Ellas, National consortium». Transnational Access to large scale synchrotron radiation facilities in the frame of “BioStruct-X” April 2012-2015 (coordinator: Dr. E.D. Chrysina) (users’ visits to Synchrotron Radiation Source-EU Large scale facilities with accommodation and travel expenses)
  • «REN-ATHENS» Researchers’ Night: meeting science and your next door scientist, Contract no. 633335 Horizon2020 (Coordinator: Dr G. Fanourakis/ NCSR-Demokritos, PI for NHRF, ED Chrysina, budget for NHRF €11.200)
  • «Advancement of Research Capability for the Development of New Functional Compounds - ARCADE». FP7-REGPOT-2009-1-245866 Coordination and Support actions (Support) (2008-2013) (Coordinator: Dr. B.Steele/IOPC-NHRF) (2.966.510 €) (01.12.2009-30.05.2013) (ED Chrysina budget: €180,000)
  • «Synthesis, kinetic and crystallographic studies of potential inhibitors targetin glycogen phosphorylase », (2010-2013) HERAKLEITOS ΙΙ (in cooperation with Assoc. Prof. Th. Gimisis (PI) and Prof. D. Kyriakidis, former president of NHRF)
  • «Molecular, structural and catalytic studies of novel biocatalysts (hemicelluloses) implicated in plant biomass degradation», (2010-2013) HERAKLEITOS ΙΙ (in cooperation with Prof. P. Christakopoulos/NTUA (PI) and Prof. D. Kyriakidis, former president of NHRF)
  • Funding for access to large scale facilities for performing experimental work, EMBL/DESY Hamburg unit for use of the synchrotron radiation source (2011) (P.I. Dr E.D. Chrysina).
  • Funding for access to large scale facilities for performing experimental work (Trans-national Access award), Protein Production Platform P-CUBE for optimizing human brain glycogen phosphorylase expression (2011) (P.I. Dr E.D. Chrysina).
  • Funding for access to large scale facilities for performing experimental work, EMBL/DESY Hamburg unit for use of the synchrotron radiation source (2009-2010) (P.I. Dr E.D. Chrysina).
  • Funding for access to large scale facilities, SRS-Daresbury Laboratory for use of the synchrotron radiation source on “Determining the structure of a new alkaline xylanase III (F/10) from Fusarium Oxysporum” (P.I. Dr E.D. Chrysina, 2007).

 

Research Funding (2001 - 2008)

(in co-operation N.G. Oikonomakos, head of the Structural Biology & Chemistry Group & Director of the Institute of Organic and Pharmaceutical Chemistry (IOPC/NHRF)

  • «European consolidation and promotion of research capacity in the area of structure-based drug discovery – EUROSTRUCT». FP7-REGPOT-2008-230146 Coordination and Support actions (Support) (2008-2011) (Coordinator: N.G Oikonomakos/ D.D. Leonidas, 2008-2011/S.E. Zographos, 2011-2012) (€986.000).
  • «Drug design at the molecular level using approximate and exact computational methods- DRUGDESI» Transfer of Knowledge (ToK) Marie Curie Program, MTKD-CT-2006-042776 (2007-2011) (Coordinator: N.G. Oikonomakos/ E.D. Chrysina Sept. 2008-11) (€186.396).
  • «A European Research Training Site for the Design and Synthesis of Novel Neuroprotective and Hypoglycaemic Agents through a Multi-disciplinary approach», EURODESY, EU Marie Curie Early Stage Training (EST) network, MEST-CT-020575 (Coordinator: Dr N.G. Oikonomakos, with the participation of all research groups of IOPC/NHRF and Molecular Endocrinology group o IBRB/NHRF (2006-2010), €984,122.
  • Structure-based design of new hypoglycaemic drugs, Κοινό Διακρατικό Πρόγραμμα μεταξύ Ελλάδος-Ουγγαρίας (Joined Research and Technology Project between Greece-Hungary)/ GSRT PI Dr N.G. Oikonomakos/NHRF and Prof. Lászlo Somsák/Department of Organic Chemistry, University of Debrecen, Debrecen, Hungary (2004-2006) (€11.740)
  • Discovery of small-molecule inhibitors of glycogen phosphorylase, as potential hypoglycaemic agents, guided by structure-based drug design and in silico virtual ligand screening, Scientific and Technological cooperation between Greece and USA/GSRT, to Dr. N.G. Oikonomakos/NHRF and Prof. Alexander D. MacKerell, Jr., Computer-Aided Drug Design Center, School of Pharmacy, Univ. of Maryland, Baltimore, MD, USA) (2004-2006) (€60.000).
  • Crystallisation and preliminary crystallographic studies on human phosphoenolpyruvate carboxykinase, a molecular target for types 2 diabetes therapy. Supported by Pfizer Inc. (2003-2005) (PI. Dr. N.G. Oikonomakos/NHRF) (€8.000).
  • Exploitation of the scientific results obtained through a spin off company dedicated to structure-based drug design. 02 PRAXE3/GSRT (PI Dr N.G. Oikonomakos/NHRF (2003-2004) (€44.000).
  • Glycogen phosphorylase as molecular target for the development of new potent hypoglycaemic drugs, PENED 2001/GSRT (in collaboration with Prof. C. Sakarellos/Dept. of Chemistry, Univ. of Ioannina, Ioannina, Greece, coordinator Dr N.G. Oikonomakos/NHRF, Business partner Aventis Pharma Deutsland GmbH) (2002-2005), €128,187.82
  • Calmodulin regulated protein kinases: molecular and cellular functions of five prototypic members from the human genome by a multidisciplinary approach. HPRN-CT-2002-00252/EU Research Network (Coordinator Matthias Wilmanns, EMBL, Hamburg Outstation; participants Louise Johnson, Univ. of Oxford, Adi Kimchi, Weizmann Inst. of Science, Mathias Gautel, King’s College London, UK, N.G. Oikonomakos (PI), NHRF, Mauno Vihinen, Univ. of Tampere) (2002-2006) (€200.000).
  • Inhibitors of glycogen phosphorylase and phosphorylase kinase as potential antidiabetic drugs, ENTER/GSRT, 2002-2004 to Dr E.D. Chrysina (Reintegrated researcher from abroad) and Dr N.G. Oikonomakos/NHRF (Host). Business partner AstraZeneca UK Ltd), Total Budget € 73.250,00.
  • European Structural Biology Forum (ESBF)-to a European infrastructure cooperation network/EU (2001-2005). An Infrastructure Cooperation Network (coordinated by Prof. Rolf Hilgenfeld, P.I. Dr. N.G. Oikonomakos/NHRF) with 10 European Research Infrastructures, 8 Users, and Bruker GmbH, Karlsruhe, Germany, Travel grants (€11.200)
  • Glycogen phosphorylase inhibitors as potential antidiabetic drugs. A Royal Society UK- Joint Project Grant to Prof. Dame L.N. Johnson, FRS/Laboratory of Molecular Biophysics, University of Oxford and Dr. N.G. Oikonomakos/NHRF. (2001-2003) (£12,000).
  • Molecular recognition and rational design of potential drugs, Joint Research and Technology Project between Greece-Cyprus/GSRT to Dr. N.G. Oikonomakos/NHRF and Prof. G. Archontis/University of Cyprus, Cyprus (2001-2003), € 8,804.11
  • Crystallisation studies and crystallographic studies of glycogen phosphorylase. Supported by AstraZeneca, UK (PI Dr N.G. Oikonomakos, Researcher Dr ED Chrysina) (2001-2003) £50,000.
  • Crystallographic studies of glycogen phosphorylase-inhibitor complexes. Supported by Aventis Pharma Deutschland GmbH (Frankfurt am Main, Germany) (PI Dr N.G. Oikonomakos /NHRF) (2001) (DM15.000).
  • From enzyme-inhibitor complex crystal structure to the design of potential hypoglycaemic drugs for the treatment of non-insulin-dependent diabetes mellitus, Joined Research and Technology Project between Greece-Hungary/GSRT to Dr N.G. Oikonomakos/NHRF and Prof. L. Somsak/Department of Medicinal Chemistry, University of Debrecen, Debrecen, Hungary (1999-2001) (€11.738,81)
  • Structure-Function relation of muscle allosteric proteins: molecular recognition of small molecules by proteins and structure-based drug design. PENED 1999/GSRT. (PI N.G. Oikonomakos / NHRF), (1999-2001) (€13.2061,63)
  • Structure/function relationships in enzymes of glycogen metabolism, Wellcome Trust Biomedical Research Collaboration Grant (with the Laboratory of Molecular Biophysics, Univ. of Oxford) (1997-2001) (£12,000).

 

Other research grants

  • «Instruct-EL: a National Research Infrastructure on Integrated Structural biology» involving 14 academic & research organizations across the country (P.I. Dr E.D. Chrysina).
  • Infrastructure for NMR, EM and X-ray crystallography for translational research, i-NEXT”. Proposal No. SEP-210169978, Horizon2020-INFRAIA-2014-2015 (Integrating and operating existing national and regional research infrastructures of European interest, Research and Innovation Action-RIA) (Coordinator: Prof. Rolf Boelens, ED Chrysina, member of the Greek partners from Univ. of Patras)
  • «GPdia – A multidisciplinary investigation towards inhibitors of glycogen phosphorylase designed and evaluated as antidiabetic molecules» French Agence Nationale de la Recherche (ANR) (coordinator: Prof. Jean-Pierre Praly, Université Claude-Bernard Lyon 1).
  • «Molecular, structural study study of novel biocatalysts (hemicelluloses) that were isolated με with the aid of bioinformatics tools» PEVE 2008 (P.I. Prof. P. Christakopoulos, NTUA).

 

Service provision to users of the facilities available at ICB/NHRF

  • X-ray Diffraction Analyses and analyses of bioactive compounds (XRPD, XRD) for pharmaceutical companies, as services to external users (2016 – present) (Scientist in charge: Dr E.D. Chrysina)

 

Publications

Google scholar: https://scholar.google.com/citations?hl=en&user=hUTwx40AAAAJ
Orcid: https://orcid.org/0000-0001-8147-9030

Publications in peer-reviewed journals (*corresponding author)

Front Covers in peer-reviewed journals

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2022

2017

2011

2011

 

         

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2006

2005

2003

2003

2002

 

2022-2018

2022

  • Minadakis, M-P., Mavreas, K.F., Neofytos, D.D., Paschou, M., Kogkaki A., Athanasiou, V., Mamais, M., Veclani, D., Iatrou, H., Venturini, A., *Chrysina, E.D., Papazafiri, P., Gimisis, T. (2022) A glucose-based molecular rotor inhibitor of glycogen phosphorylase as a probe of cellular enzymatic function. Org. Biomol. Chem. 20, 2407-2423 (IF2021-22 3.876) (Front Cover)
    PDB ID: 7Q5I
  • Neophytos, D.D., Papagiannopoulos, A., *Chrysina, E.D., Pispas, S. (2022) Formation and physicochemical properties of Glycogen Phosphorylase in complex with a cationic polyelectrolyte Int. J. Biol. Macromol. 206  371-380 (IF2021 6.953)

2021

  • Chontzopoulou, E., Papaemmanouil, C.D., Chatziathanasiadou, M.V., Kolokouris, D., Kiriakidi, S., Konstantinidi, A., Gerogianni, I., Tselios, T., Kostakis, I.K., Chrysina, E.D., Hadjipavlou-Litian, D., Tzeli, D., Tzakos, A.G., Mavromoustakos, T. (2021) Molecular investigation of artificial and natural sweeteners as potential anti-inflammatory agents. J. Biomol. Struct Dyn. 9, 1-13 (IF2020 3.392)

2020

  • Dimarogona, M., Topakas, E., Christakopoulos, P. & *Chrysina, E. D. (2020) The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family. FEBS Lett. 1873-3468.13776 (IF2020 6.750)
    PDB ID: 5MEM
  • Zarafeta, D., Galanopoulou, A.P., Leni, M.E., Kaili, S.I., Chegkazi, M.S., Chrysina, E.D., Kolisis, F.N., Hatzinikolaou, D.G., Skretas, G. (2020) XynDZ5: A New Thermostable GH10 Xylanase. Front. Microbiol. 11, Article 545(IF2020 4.190)
  • Mavreas, K.F., Neofytos, D.D., *Chrysina, E.D., Venturini, A., Gimisis, T. (2020) Synthesis, kinetic and conformational studies of 2-substituted-5-(β-D-glucopyranosyl)-pyrimidin-4-ones as potential inhibitors of glycogen phosphorylase. Molecules 25, Article 5463 (IF2020 3.010)

2019

  • Maffeis, V., Mavreas, K., Monti, F., Mamais, M., Gustavsson, T., Chrysina, E.D., Markovitsi, D., Gimisis, T., Venturini, A. (2019) Multiscale time-resolved fluorescence study of a glycogen phosphorylase inhibitor combined with quantum chemistry calculations. Phys. Chem. Chem. Phys. 21, 7685-7696 (IF2019 3.43)
  • Stathi, A., Mamais, M., Chrysina, E.D., Gimisis, T. (2019) Anomeric spironucleosides of β-D-glucopyranosyl uracil as potential inhibitors as glycogen phosphorylase. Molecules 24, 2327-2337 (IF2019 3.267)
  • Chountoulesi M., Pippa N., Chrysostomou V., Pispas S., Chrysina E.D., Forys A., Otulakowski L., Trzebicka B. and Demetzos C. (2019) Stimuli-responsive lyotropic liquid crystalline nanosystems with incorporated poly(2-dimethylamino ethyl methacrylate)-β-poly(lauryl methacrylate) amphiphilic block copolymer. Polymers 11, 1400-1424 (IF2019 3.426)

2018

  • Chountoulesi, M., Pippa, N., Pispas, S., Chrysina, E.D., Forys, A., Trzebicka, B., Demetzos, C. (2018). Cubic lyotropic liquid crystals as drug delivery carriers: Physicochemical and morphological studies. Int. J. Pharm. 550, 57-70 (IF2018 4.213)
2017-2013

2017

  • Mamais, M., Esposti, A.D. Kouloumoundra, V., Gustavsson, T., Monti, F., Venturini, A., *Chrysina, E.D., Markovitsi, D., Gimisis, T. (2017). A New Potent Inhibitor of Glycogen Phosphorylase Reveals the Basicity of the Catalytic Site. Chemistry- A European Journal 23, 8800-8805 (IF2017 5.16) (Frontipiece)
    PDB ID: 5MEM

2016

  • Goyard, D. Kónya, B, Chajistamatiou, A.S., Chrysina, E.D., Leory, J., Balzarin, S., Tournier, M., Tousch, D., Petit, P., Duret, C., Maurel, P., Somsák, L., Docsa, T., Gergely, P., Praly, J.P., Azay-Milhau, J., Vidal, S. (2016) Glucose-derived spiro-isoxazolines are anti-hyperglycemic agents against type 2 diabetes through glycogen phosphorylase inhibition. Eur. J. Med. Chem. 108, 444-454. (IF2016 4.519)
  • Louros, N.N., Chrysina, E.D., Baltatzis, G.E., Patsouris, E.S., Hamodrakas, S.J., Iconomidou, V.A. (2016) A common “aggregation-prone” interface possibly participates in the self-assembly of human zona pellucida proteins. FEBS Lett. 590, 619-630. (IF2016 3.9)
  • Galal, S.A., Khattab, M., Andreadaki, F., *Chrysina, E.D., Praly, J.P., Ragab, F.A., El Diwani, H.I. (2016). Synthesis of (benzimidazol-2-yl)aniline derivatives as glycogen phosphorylase inhibitors. Biorg. Med. Chem. 24, 5423-5430 (IF2016 3.129)
  • Zarafeta, D., Szabo, Z., Moschidi, D., Phan, H., Chrysina, E.D., Peng, Xu, Ingham, C.J., Kolisis, F.N. and Skretas, G. (2016). EstDZ3: a new esterolytic enzyme exhibiting remarkable thermostability. Front. Microbiol. 7, Article 1779 (IF2015 4.165)
  • Zarafeta, D., Moschidi, D., Ladoukakis, E., Gavrilov, S., Chrysina, E.D., Chatziioannou, A., Kublanov, I., Skretas, G., Kolisis, F.N. (2016). Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases. Sci. Rep. 6-38886 1-16 (IF2016 4.62)

2015

  • Mavrokefalos, N., Myrianthopoulos, V., Chajistamatiou, A.S., Chrysina, E.D., Mikros, E. (2015) Discovery of the glycogen phosphorylase-modulating activity of a resveratrol glucoside by using a virtual screening protocol optimized for solvation effect. Planta Med. 81, 507-516. (IF2015 2.152)
  • Mamais, M., Kouloumoundra, V., Smyrli, E., Grammatopoulos, P., Chrysina, E.D., Gimisis, T. (2015) Synthesis of N4-aryl-β-D-glucopyranosylcytosines: a methodology study. Tetrahedron Lett. 56, 5549-5552 (IF2015 2.347)

2014

  • Louros, N.N., Iconomidou, V.A., Tsiolaki, P.L., Chrysina, E.D., Baltatzis, G.E., Patsouris, E.S., Hamodrakas, S.J. (2014) An N-terminal natiuretic peptide (NT-proANP) ‘Aggregation-Prone’ segment involved in isolated atrial amyloidosis. FEBS Lett. 588, 52-57. (IF2014 3.169)
  • Czifrák, K., Páhi, A., Deák, S., Kiss-Szikszai, A., Kövér, K.E., Docsa, T, Gergely, P., Alexacou, K-M., Papakonstantinou, M., Leonidas, D.D., Zographos, S.E., *Chrysina, E.D., Somsák, L. (2014) Glucopyranosylidene-spiro-iminothiazolidinone, a new bicyclic ring system: synthesis, derivatization, and evaluation for inhibition of glycogen phosphorylase by enzyme kinetic and crystallographic methods. Bioorg. Med. Chem. 22, 4028-4041. (IF2014 2.793)
  • Goyard, D., Chajistamatiou, A.S., Sotiropoulou, A.I., *Chrysina, E.D., Praly, J-P., Vidal, S. (2014) Efficient atropodiastereoselective access to 5,5'-bis-1,2,3-triazoles: studies on 1-glucosylated 5-halogeno 1,2,3-triazoles and their 5-substituted derivatives as glycogen phosphorylase inhibitors. Chemistry-A European Journal 20, 5423-5432. (IF2014 5.731)

2013

  • Charavgi, M., Dimarogona, M., Topakas, E., Christakopoulos, P., *Chrysina, E.D. (2013) The crystal structure of a novel glucuronoyl esterase from Myceliophthora thermophila gives new insights on its role as a potential biocatalyst. Acta Crystallogr. D69, 63-73. (IF2013 7.232)
    PDB IDs: 4G4G, 4G4I, 4G4J
2012-2008

2012

  • Azay-Milhau, J., Duret, C., Vidal, S., Goyard, D., Balzarin, S., Leroy, J., Tournier, M., Tousch, D., Ferrare, K., Maurel, P., Chrysina, E. D., Petit, P., Praly, J.P. (2012) A glycogen phosphorylase inhibitor developed as a potential antidiabetic compound, Fundamental & Clinical Pharmacology 26 S1, 94. (IF2012 1.78)
  • Nagy, V., Felföldi, N., Kónya, B., Praly, J.-P., Docsa, T., Gergely, P., *Chrysina, E.D., Tiraidis, C., Kosmopoulou, M.N., Alexacou, K.-M., Konstantakaki, M., Leonidas, D.D., Zographos, S.E., Oikonomakos, N.G., Kozmon, S., Tvaroška,I., Somsák, L. (2012) N-(4-Substituted-benzoyl)-N'-(β-D-glucopyranosyl)ureas as inhibitors of glycogen phosphorylase: synthesis and evaluation by kinetic, crystallographic, and molecular modelling methods Bioorg. Med. Chem. 20, 1801-1816. (IF2012 2.903)
    PDB IDs: 2QNB, 2QLM, 2QLN, 2QN3, 2QN7, 2QN8, 2QN9
  • Dimarogona, M., Topakas, E., Christakopoulos, P., *Chrysina, E.D. (2012) The structure of a GH10 xylanase from Fusarium oxysporum reveals the presence of an extended loop on top of the catalytic cleft Acta Crystallogr. D68, 735-742. (IF2012 14.103)
    PDB IDs:
    3U7B
  • Iconomidou, V.A., Giannelou, P.K., Leontis, A., Chrysina, E.D., Hamodrakas, S.J. (2012) Identification of a novel amyloidogenic peptide in the sequence of the highly amyloidogenic Human Calcitonin J. Peptide Sci. 18, S1, S162. (IF2012 2.071)
  • Feuillastre, S., Chajistamatiou, A.S., Potamitis, C., Zervou, M., Zoumpoulakis, P., *Chrysina, E.D., Praly, J-P., Vidal, S. (2012) C-glucosylated malonitrile as a key intermediate towards carbohydrate-based glycogen phosphorylase inhibitors Bioorg. Med. Chem., 20, 5592-5599. (IF2012 2.903)
  • Goyard, D., Baron, M., Skourti, P.V., Chajistamatiou, A.S., Docsa, T., Gergely, P., *Chrysina, E.D., Praly, J.-P., Vidal., S. (2012) Synthesis of 1,2,3-triazoles from azido xylose and 5-thioxylose: Evaluation of the xylose scaffold for the design of potential glycogen phosphorylase inhibitors Carbohydrate Res. 364, 28-40. (IF2012 2.044)

2011

  • Kun, S., Nagy, G.Z., Tóth, M, Czecze L., Van Nhien, A.N., Docsa, T., Gergely, P., Charavgi, M.D., Skourti, P.V., Chrysina, E.D., Patonay, T., Somsák, L. (2011) Synthesis of variously coupled conjugates of D-glucose, 1,3,4-oxadiazole, and 1,2,3-triazole for inhibition of glycogen phosphorylase. Carbohydrate Res. 346, 1427-1438. (IF2011 2.332)
  • *Chrysina, E.D., Chajistamatiou, A., Chegkazi, M. (2011) From structure-based to knowledge-based drug design through x-ray protein crystallography: sketching glycogen phosphorylase binding sites. Curr. Med. Chem. 18, 2620-2629. (IF2011 4.94) Front Cover.
  • Alexacou, K.-M., Zhang, Y.Z., Praly, J.-P., Zographos, S.E., Chrysina, E.D., Oikonomakos, N.G., Leonidas, D.D. (2011). Halogen-substituted (C-β-D-glucopyranosyl)-hydroquinone regioisomers: Synthesis, enzymatic evaluation and their binding to glycogen phosphorylase. Bioorg. Med. Chem. 19, 5125-5136. (IF2011 2.921)
    PDB IDs: 3NP9, 3NP7, 2NPA, 3S0J

2010

  • Zhong, S.J., Alexacou, K.M., Chara vgi, M.A., Tiraidis, C., Zographos, S.E., Leonidas, D.D, Chrysina, E.D., Oikonomakos, N.G., MacKerell, A.D. (2010). Glycogen phosphorylase inhibitor identification targeting the inhibitor binding site using virtual screening and experimental validation. Abstracts of Papers of the Amer. Chem. Soc., 240, 265-COMP
  • Felfoldi, N., Toth, M., Chrysina, E.D., Charavgi, M.-D., Alexacou, K.-M., Somsak, L., (2010). Synthesis of new glycosyl biuret and urea derivatives as potential glycoenzyme inhibitors. Carbohydrate Res. 345, 208-213. (IF2010 2.22)
  • Alexacou, K.M., Tenchiu Deleanu, A.C., Chrysina, E.D., Charavgi, M.D., Kostas, I.D., Zographos, S.E., Oikonomakos, N.G., Leonidas, D.D. (2010). The binding of β-D-glucopyranosyl-thiosemicarbazone derivatives to glycogen phosphorylase: A new class of inhibitors. Bioorg. Med. Chem. 18, 7911-7922. (IF2010 2.978)
    PDB IDs: 3NC4, 3MRV, 3MT8, 3MT9, 3MRT, 3MT7, 3MRX, 3MS2, 3MS4, 3MS7, 3MQF, 3MTA, 3MTD, 3MTB, 3MSC, 3NP9, 3NP7, 2NPA, 3S0J
  • *Chrysina, E.D. The prototype of glycogen phosphorylase (2010) Mini Rev. Med. Chem. 10, 1093-1101. (IF2010 3.03)

2009

  • *Chrysina, E.D., Bokor, E., Alexacou, K-M., Charavgi, M-D., Oikonomakos, G.N., Zographos, S.E., Leonidas, D.D., Oikonomakos, N.G., *Somsák, L. (2009). Amide-1,2,3-triazole bioisosterism: the glycogen phosphorylase case. Tetrahedron Asymmetry 20, 733-740. (IF2009 2.625)
    PDB IDs: 3G2I, 3G2H, 3G2K, 3G2J, 3G2L, 3G2N
  • Benltifa, M., Hayes, J., Vidal., S. Gueyrard, D., Goekjian, P.G., *Praly, J-P, Kizilis, G., Tiraidis, C., Alexacou, K-M., *Chrysina, E.D., Zographos, S.E., Leonidas, D.D., Archontis, G., Oikonomakos, N.G. (2009). Glucose-based spiro-isoxazolines: A new family of potent glycogen phosphorylase inhibitors. Bioorg. Med. Chem. 17, 7368-7380. (IF2009 2.822)
    PDB IDs: 2QRM, 2QRQ, 2QRP, 2QRH, 2QRG

2008

  • Alexacou, K.M., Hayes, J.M., Tiraidis, C., Zographos, S.E., Leonidas, D.D., Chrysina, E.D., Archontis, G., Oikonomakos, N.G., Paul, J.V., Varghese, B., Loganathan, D. (2008). Crystallographic and computational studies on 4-phenyl-N-(b-D-glucopyranosyl)-1H-1,2, 3-triazole-1-acetamide, an inhibitor of glycogen phosphorylase: Comparison with a-D-glucose, N-acetyl-β-D-glucopyranosylamine and N-benzoyl-N'-β-D-glucopyranosyl urea binding. Proteins: Structure, Function, and Bioinformatics 71, 1307-23. (IF2008 3.419)
    PDB IDs: 2PYD, 2PYI
  • Dimarogona, M., Topakas, E., Christakopoulos, P., *Chrysina, E.D. (2008). Structural studies of a new alkaline xylanase (FoXyn10a) from Fusarium oxysporum F3. FEBS J. 275, 161. (IF2008 3.139)
  • Somsák, L., Czifrák, K., Tóth, M., Bokor, E., *Chrysina, E.D., Alexacou, K.M., Hayes, J.M., Tiraidis, C., Lazoura, E., Leonidas, D.D., Zographos, S.E., Oikonomakos, N.G. (2008). New Inhibitors of Glycogen Phosphorylase as Potential Antidiabetic Agents. Curr. Med. Chem. 15, 2933-2983. (IF2008 4.823)
2007-1996

2007

  • He, L., Zang, Y.Z., Tanoh, M., Chen, G.-R., Praly, J.-P., Chrysina, E.D., Tiraidis, C., Kosmopoulou, M.N., Leonidas, D.D. and Oikonomakos, N.G. (2007). In the search for glycogen phosphorylase inhibitors: synthesis of C-D-glycopyranosyl-hydroquinones and -benzoquinones. Inhibition of and binding to glycogen phosphorylase in the crystal. Eur. J. Org. Chem. 596-606. (IF2007 2.914)
    PDB IDs: 2FET, 2FF5

2006

  • Petsalakis, E.I., Chrysina, E.D., Tiraidis, C., Hadjiloi, T., Leonidas, D.D., Oikonomakos, N.G., Aich, U., Varghese, B. and Loganathan, D. (2006). Crystallographic studies on N-azidoacetyl-b-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-b-D-glucopyranosylamine. Bioorg Med Chem, 5316-5324. (IF2006 2.624)
    Pdb ID: 2FFR
  • Hadjiloi, T., Tiraidis, C., Chrysina, E.D., Leonidas, D.D., Oikonomakos, N.G., Tsipos, P. and Gimisis, T. (2006). Binding of oxalyl derivatives of b-D-glucopyranosylamine to muscle glycogen phosphorylase b. Bioorg. Med. Chem., 3872-3882. (IF2005 2.286) Front Cover
    Pdb Ids: 2F3P, 2F3Q, 2F3S, 2F3U
  • Anagnostou, E., Kosmopoulou, M.N., Chrysina, E.D., Leonidas, D.D., Hadjiloi, T., Tiraidis, C., Györgydeák, Z., Somsák, L., Docsa, T., Gergely, P., Kolisis, F.N., and Oikonomakos, N.G. (2006) Crystallographic studies on two bioisosteric analogues, N-acetyl-b-D-glucopyranosylamine and N-trifluoracetyl-b-D-glucopyranosylamine, potent inhibitors of muscle glycogen phosphorylase. Bioorg. Med. Chem. 14, 181-189. (IF2005 2.286) Front Cover
    Pdb Ids: 1WW2, 1WW3

2005

  • Chrysina, E.D., Kosmopoulou, M.N., Kardakaris, R., Bischler, N., Leonidas, D.D., Kannan, T., Loganathan, D. and Oikonomakos, N.G (2005). Binding of b-D-glucopyranosyl bismethoxyphosphoramidate to glycogen phosphorylase b: kinetic and crystallographic studies. Bioorg. Med. Chem. 13, 765-772. (IF2005 2.286)
    Pdb Id: 1XC7
  • Chrysina, E.D., Kosmopoulou, M.N., Tiraidis, C., Kardakaris, R., Bischler, N., Leonidas, D.D., Hadady, Z., Somsak, L., Docsa, T., Gergely, P. and Oikonomakos, N.G. (2005). Kinetic and crystallographic studies on 2-(b-D-glucopyranosyl-5-methyl-1, 3, 4-oxadiazole, –benzothiazole, and –benzimidazole, inhibitors of muscle glycogen phosphorylase b. Evidence for a new binding site. Protein Sci. 14, 873-888. (IF2005 3.618)
    Pdb Ids: 1XKX, 1XL0, 1XL1
  • Kosmopoulou, M.N., Oikonomakos, N.G., Chrysina, E.D., Leonidas, D.D., Kostas, I.D., Wendt, K.U., Klabunde, T., Defossa, E. (2005) Crystallographic studies on acyl ureas, a new class of inhibitiors of glycogen phosphorylase. Acta Crystallogr. A61, C182
  • Oikonomakos, N.G., Kosmopoulou, M.N., Chrysina, E.D., Leonidas, D.D., Kostas, I.D., Wendt, K.U., Klabunde, T., and Defossa, E. (2005) Crystallographic studies on acyl ureas, a new class of glycogen phosphorylase inhibitors, as potential antidiabetic drugs. Protein Sci. 14, 1760-1771. (IF2005 3.618)
    Pdb Ids: 1WUT, 1WUY, 1WV0, 1WV1
  • Archontis, G., Watson, K.A., Xie, Q., Andreou, G., Chrysina, E.D., Zographos, S.E., Oikonomakos, N.G., and Karplus, M. (2005) Glycogen phosphorylase inhibitorsQ A free energy perturbation analysis of glucopyranose spirohydantoin analogues. Proteins: Structure, Function, and Bioinformatics 61, 984-998. (IF2005 4.684)
  • =Watson, K.A., =Chrysina, E.D., Tsitsanou K.E., Zographos, S.E., Gregoriou, M., Archontis, G., Fleet, G.W.J., and Oikonomakos, N.G. (2005) Kinetic and crystallographic studies of glucopyranose spirohydantoin and glucopyranosylamine analogues inhibitors of glycogen phosphorylase. Proteins: Structure, Function, and Bioinformatics 61, 966-983. (=Equal Contribution) (IF2005 4.684)
    Pdb Ids: 1FS4, 1FTQ, 1FTW, 1FTY, 1FU4, 1FU7, 1FU8
  • Kosmopoulou, M.N., Leonidas, D.D., Chrysina, E.D., Eisenbrand, G., and Oikonomakos, N.G. (2005) Indirubin-3'-aminooxy-acetate inhibits glycogen phosphorylase by binding at the inhibitor and the allosteric site. Broad specificities of the two sites. Letters in Drug Design & Discovery 2, 377-390. (IF2005 0.57) Front Cover
    Pdb Id: 1Z62

2004

  • Kosmopoulou, M.N., Leonidas, D.D., Chrysina, E.D., Bischler, N., Eisenbrand, G., Sakarellos C.E., Pauptit, R. and Oikonomakos, N.G. (2004). Binding of the potential antitumour agent indirubin-5΄-sulphonate at the inhibitor site of rabbit muscle glycogen phosphorylase b. Comparison with ligand binding to pCDK2-cyclin A complex. Eur. J. Biochem. 271, 2280-2290. (IF2004 3.22)
    Pdb Id: 1UZU
  • Ganotidis, M.H., Elemes, Y., Alexopoulos, C., Sakarellos, C., Kosmopoulou, M.N., Chrysina, E.D., Leonidas, D.D., Oikonomakos, N.G. (2004). Glycogen phosphorylase inhibitors as potential drugs for the treatment of type 2 diabetes. J. Peptide Sci.  10, S2, Supplement: 3rd International and 28th European Peptide Symposium, September 5–10, 2004; Prague, Czech Republic, 244-244. (IF2004 1.57)
  • Skouridou, V., Chrysina, E.D., Stamatis, H., Oikonomakos, N.G. and Kolisis, F.N. (2004). Kinetic and modelling studies on the lipase catalysed enantioselective esterification of (±)-perillyl alcohol. J. Mol. Catal. B-Enzym. 29, 9-12. (IF2004 1.42)

2003

  • Leonidas, D.D., Swamy, B.M., Bhat, A.G., Inamdar, S.R., Kosmopoulou, M.N., Chrysina, E.D. and Oikonomakos, N.G. (2003). Crystallization and preliminary X-ray crystallographic analysis of Sclerotium rolfsii lectin. Acta Crystallogr. D59, 363-365. (IF2003 2.208)
  • Oikonomakos, N.G., Chrysina, E.D., Kosmopoulou, M.N. and Leonidas, D.D. (2003). Crystal structure of rabbit muscle glycogen phosphorylase a in complex with a potential hypoglycaemic drug at 2.0 Å resolution. BBA-Proteins-Proteom. 1647, 325-332. (IF2003 1.602)
    Pdb Ids: 1LWN, 1LWO
  • Pinotsis, N., Leonidas, D.D., Chrysina, E.D., Oikonomakos, N.G. and Mavridis, I.M. (2003). The binding of b- and g-cyclodextrins to glycogen phosphorylase b: Kinetic and crystallographic studies. Protein Sci. 12, 1914-1924. (IF2003 3.787) Front Cover
    Pdb Ids: 1P29, 1P2B, 1P2D, 1P2G
  • Leonidas, D.D., Chavali, G.B., Oikonomakos, N.G., Chrysina, E.D., Kosmopoulou, M.N., Vlassi, M, Frankling, C. and Acharya, K.R. (2003). High-resolution crystal structures of ribonuclease A complexed with adenylic and uridylic nucleotide inhibitors. Implications for structure-based design of ribonucleolytic inhibitors. Protein Sci. 12, 2559-2574. (IF2003 3.787) Front Cover
    Pdb Ids: 1O0F, 1O0H, 1O0M, 1O0N, 1O0O
  • Chrysina, E.D., Oikonomakos, N.G., Zographos, S.E., Kosmopoulou, M.N., Bischler, N., Leonidas, D.D., Kovács, L., Docsa, T., Gergely, P. and Somsák, L. (2003). Crystallographic studies on a- and b-D-glucopyranosyl formamide analogues, inhibitors of glycogen phosphorylase. Biocatal. Biotransfor. 21, 233-242. (IF2003 1.085)
    Pdb Ids: 1P4G, 1P4H, 1P4J

2002

  • Oikonomakos, N.G., Kosmopoulou, M., Zographos, S.E., Leonidas, D.D., Chrysina, E.D., Somsák L., Nagy, V., Praly, J.P., Docsa, T., Tóth, A. and Gergely, P. (2002). Binding of N-acetyl-N΄-b-D-glucopyranosyl urea and N-benzoyl-N΄-b-D-glucopyranosyl urea to glycogen phosphorylase b- Kinetic and crystallographic studies. Eur. J. Biochem. 269, 1684-1696. (IF2002 2.999) Front Cover
    Pdb Ids: 1K06, 1K08, 1KTI
  • Cook, A., Lowe, E.D., Chrysina, E.D., Skamnaki, V.T., Oikonomakos, N.G. and Johnson, L.N. (2002) Structural studies on phospho-CDK2/cyclin A bound to nitrate, a transition state analogue: Implications for the protein kinase mechanism. Biochemistry 41, 7301-7311. (IF2002 4.064)
    Pdb Id: 1GY3

2001

  • Greene, L.H., Chrysina, E.D., Irons, L.I., Papageorgiou, A.C., Acharya, K.R. and Brew K. (2001). Role of conserved residues in structure and stability: Tryptophans of human serum retinol-binding protein, a model for the lipocalin superfamily Protein Sci. 10, 2301-2316. (IF2001 3.472)
    Pdb Ids: 1JYD, 1JYJ

2000

  • Chrysina, E.D., Brew, K. and Acharya, K.R. (2000).Crystal structures of apo- and holo-bovine a-Lactalbumin at 2.2 Å resolution reveal an effect of calcium on inter-lobe interactions. J. Biol. Chem. 275, 37021-37029. (IF2000 6.62)
    PDB IDs: 1F6R, 1F6S

1999

  • Chrysina, E.D., Brew, K., Acharya, K.R. (1999). Structural studies on bovine α-Lactalbumin. Acta Crystallogr. A55, 367, Sup.5, P12.04.134

1997

  • Zographos, S.E., Oikonomakos, N.G., Tsitsanou, K.E., Leonidas, D.D., Chrysina, E.D., Skamnaki, V.T., Bischoff, H., Goldmann, S., Watson, K.A. and Johnson, L.N. (1997). The structure of glycogen phosphorylase b with an alkyl-dihydropyridine-dicarboxylic acid compound, a novel and potent inhibitor. Structure 5, 1413-1425. (IF1997 7.633)
    PDB ID: 2AMV

1996

  • Brandstetter, T.W., delaFuente, C., Kim, Y.H., Johnson, L.N., Crook, S., Lilley, P.M.D.Q., Watkin, D.J., Tsitsanou, K.E., Zographos, S.E., Chrysina, E.D., Oikonomakos, N.G. and Fleet, G.W.J. (1996). Glucofuranose analogues of hydantocidin. Tetrahedron 52, 10721-10736. (IF1996 2.232)

 

Chapters in books
  • Chegkazi M.S., Mamais M., Sotiropoulou, A.I., ›Chrysina, E.D. (2018) Rational Drug Design using Integrated Structural Biology. Methods Mol Biol. 1824, 89-111, Springer Protocols.
  • Oikonomakos, N.G., Chrysina, E.D., Tiraidis, C., Leonidas, D.D., Zographos, S.E. (2007) Conformation flexibility of the 280s peptide loop in glycogen phosphorylase: implications for inhibitor design. 5th Hellenic Forum on Bioactive Peptides (P.A. Cordopatis et al., Eds.).
  • Oikonomakos, N.G., Kosmopoulou, M.N., Leonidas, D.D., Chrysina, E.D., C. Tiraidis, Bischler, N., Tsitsanou, K.E., Zographos, S.E., Kostas, I.D. and Eisenbrand, G. (2006) Indirubin and indigo analogues, as potential inhibitors of glycogenolysis: structural basis of glycogen phosphorylase inhibition. In Indirubin, the red shade of indigo, Edited by L. Meijer, N. Guyard, L. Skaltsounis and G. Eisenbrand, Editions “Life in Progress”, Station Biologique, Roscoff, France, Chapter 18, pp. 177-189.
  • Ganotidis, M.H., Elemes, Y., Alexopoulos, C., Sakarellos, C., Kosmopoulou, M.N., Chrysina, E.D., Leonidas, D.D., Oikonomakos, N.G. (2005) Glycogen phosphorylase inhibitors as potential drugs for the treatment of type 2 diabetes M. Flegel, M. Fridkin, C. Gilon, J. Slaninova (Eds.), Peptides 2004, Proceedings - BRIDGES BETWEEN DISCIPLINES, 2005, p. 844-45.
Other publications / opinions
  • Chrysina, E.D. (2021) – Interview to share the experiences of local community members of European Open Science Cloud (EOSC)
    https://ni4os.eu/2021/03/30/an-interview-with-evangelia-chrysina/

  • Diagram  Description automatically generatedAthanasiou, S., Amiridis, V., Gaviilidou, M., Gerasopoulos, E., Dimopoulos, A., Kaklamani, G., Karagiannis, F., Klampanos, I., Kondili, D., Koumantaros, K., Konstantopoulos, P., Lenaki, K., Likiardopoulos, A., Manola, N., Mitrooulou, D., Benardou, A., Boukos, N., Nousias, A., Ntaountaki, M., Oikouta, S., Ollandezou, L, Papadopoulou, E., Papastamatiou, I., Piperidis, S., Speyer, O., Tsiavos, P., Chrysina, E., Psomopoulos, F. (2020)
    DOI: 10.5281/zenodo.3908953

  • Chrysina, E., Naomi, G., Chapman S. (2019) Instruct-ULTRA D1.3 Women in Science Workshop
    DOI: 10.5281/zenodo.4304571

    Graphical user interface, website  Description automatically generated

  • Mavridis, I.M and Chrysina, E.D. (2010) Fostering Structural Biology in Greece. European Union – Issue 19, Digital Edition

 

 

 

 


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