Institute of Chemical Biology - National Hellenic Research Foundation

About Team Collaborations Funding Publications

Our group focuses on unveiling the mechanisms that affect human ageing, longevity and age-related diseases (currently focusing on Alzheimer’s disease), with the proteasome being our main target. We are interested in revealing the regulation of the proteasome and its function along with its cross-talk with other mechanisms of proteostasis. Moreover, we seek to identify natural or synthetic compounds with anti-ageing and/or anti-aggregation properties and to investigate the involved mechanisms. We mainly use the nematode C. elegans, human primary fibroblasts (the replicative senescence model) and human cells of neuronal origin. Our ultimate goal is the identification of pathways and compounds with direct application in the field of anti-ageing (nutrition, cosmetics, drugs) and anti-aggregation of proteins that characterize several proteinopathies. Finally, we take advantage of the nematode C. elegans to provide a fast toxicity testing of drugs or novel bioactive compounds in the level of a multicellular organism while respecting at the same time the 3Rs (Reduction, Replacement, Refinement).

Keywords: ageing, proteasome, proteostasis, lifespan, healthspan, testing of natural and synthetic bioactive compounds, C. elegans, replicative senescence

Current research activities

1^st Axis: Regulation of proteasome expression and function and its molecular cross-talk with other mechanisms of proteostasis

Previous work has revealed that the proteasome expression and function are down-regulated during replicative senescence and human ageing while partial proteasome inhibition results in irreversible premature senescence. We seek to reveal how the expression but also the function of the proteasome is regulated under normal but also stress conditions and upon the progression of replicative senescence, stress-induced premature senescence, ageing and age-related diseases. We investigate the positive or negative effects of various environmental factors (including bioactive compounds found in our normal diet, various kind of stresses etc) on the expression and function of the proteasome. Finally, the proteasome is one of the main actors in the maintenance of protein homeostasis (proteostasis). We aim to reveal how the proteasome cross-talks with other mechanisms of proteostasis (such as autophagy) but also with mechanisms of cellular surveillance that sense and respond to protein damage such as the heat shock response (HSR) or the Unfolded Protein Response in mitochondria (UPRmit).

Related Publications

Draganidis D., Jamurtas AZ., Chondrogianni N., Mastorakos G., Jung T., Grune T., Papadopoulos C., Papanikolaou K., Papassotiriou I., Papaevgeniou N., Poulios A., Batrakoulis, A., Deli CK., Georgakouli K., Chatzinikolaou A., Karagounis LG., Fatouros I.G. (2021) Low-grade systemic inflammation interferes with anabolic and catabolic characteristics of the aged human skeletal muscle. Oxid. Med.Cell. Longev. 8376915: 1-14.
Aktypi F., Papaevgeniou N., Voutetakis C., Chatziioannou A., Grune T., Chondrogianni N. (2021) Network analysis in aged C. elegans reveals candidate regulatory genes of ageing. Biogerontology 22: 345-367.
Binenbaum I., Lefaki M., Chondrogianni N.,* Chatziioannou A. (2020) Bioinformatic framework for analysis of transcription factor changes as the molecular link between replicative cellular senescence signaling pathways and carcinogenesis. Biogerontology 21: 357-366. (* co-corresponding author)
Sacramento Kelmer E., Kirkpatrick J.M., Mazzetto M., Baumgart M., Bartolome A., Di Sanzo S., Caterino C., Sanguanini M., Papaevgeniou N., Lefaki M., Childs D., Bagnoli S., Terzibasi Tozzini E., Di Fraia D., Romanov N., Sudmant P., Huber W., Chondrogianni N., Vendruscolo M., Cellerino A. and Ori A. (2020) Reduced proteasome activity in the aging brain results in ribosome stoichiometry loss and aggregation. Mol. Syst Biol 16(6):e9596. (cover page)
Laidou S., Alanis-Lobato G., Pribyl J., Raskó T., Tichy B., Mikulasek K., Tsagiopoulou M., Oppelt J., Kastrinaki G., Lefaki M., Singh M., Zink A., Chondrogianni N., Psomopoulos F., Prigione A., Ivics Z., Pospisilova S., Skladal P., Izsvák Z., Andrade-Navarro M.A., Petrakis S. (2020) Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery. Redox Biology 32: 101458
Papaevgeniou N., Hoehn A., Tur J.A., Klotz L.O., Grune T., Chondrogianni N. (2019) Sugar-derived AGEs accelerate pharyngeal pumping rate and increase the lifespan of Caenorhabditis elegans. Free Radic Res. 13: 1-12
Sakellari M., Chondrogianni N.*, Gonos E.S. (2019) Protein synthesis inhibition induces proteasome assembly and function. Biochem Biophys Res Commun. 514: 224-230. (* co-corresponding author)
Athanasopoulou S., Chondrogianni N., Santoro A., Asimaki K., Delitsikou V., Voutetakis K., Fabbri C., Pietruszka B., Kaluza J., Franceschi C., Gonos E.S. (2018) Beneficial effects of elderly tailored Mediterranean diet on the proteasomal proteolysis. Front. Physiol. 9: 457
Draganidis D., Jamurtas A.Z., Stampoulis T., Laschou V., Deli C.K., Georgakouli K., Papanikolaou K., Chatzinikolaou A., Michalopoulou M., Papadopoulos C., Tsimeas P., Chondrogianni N., Koutedakis Y., Karagounis L.G. and Fatouros I.G. (2018) Disparate habitual physical activity and dietary intake profiles of elderly men with low and elevated systemic inflammation. Nutrients 10 (5), pii: E566
Kapetanou M., Chondrogianni N.,* Petrakis S., Koliakos G. and Efstathios S. Gonos (2017) Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells. Free Rad. Biol. Med. 103:226-235. (* co-corresponding author)
Draganidis D., Chondrogianni N., Chatzinikolaou A., Terzis G., Karagounis L.G., Sovatzidis A., Avloniti A., Lefaki M., Protopappa M., Deli C.K., Papanikolaou K., Jamurtas A.Z., Fatouros I.G. (2017) Protein ingestion preserves proteasome activity during intense aseptic inflammation and facilitates skeletal muscle recovery in humans. Br. J. Nutr. 118: 189-200
Tsolou Α., Nelson G.; Trachana V., Chondrogianni N., Saretzki G., von Zglinicki T.; Gonos E.S. (2012) The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult. IUBMB Life 64: 432-442
Catalgol B., Ziaja I., Breusing N., Jung T., Hoehn A., Alpertunga B., Schroeder P., Chondrogianni N., Gonos E.S., Petropoulos I., Friguet B., Klotz L.O., Krutmann J. and Grune T. (2009) The proteasome is an integral part of solar ultraviolet a radiation-induced gene expression. J. Biol. Chem. 284: 30076-30086
Chondrogianni N., Trougakos I.P., Kletsas D., Chen Q.M. and Gonos E.S. (2008) Partial proteasome inhibition in human fibroblasts triggers accelerated M1 senescence or M2 crisis depending on p53 and Rb status. Aging Cell,7: 717-732. (journal’s highlight)
Stratford F.L., Chondrogianni N., Trougakos I.P., Gonos E.S. and Rivett A.J. (2006) Proteasome response to interferon-gamma is altered in senescent human fibroblasts. FEBS Lett. 580: 3989-3994
Chondrogianni N. and Gonos E.S. (2004) Proteasome inhibition induces a senescence-like phenotype in primary human fibroblasts cultures. Biogerontology 5: 55-61
Chondrogianni N., Stratford F.L.L., Trougakos I.P., Friguet B., Rivett A.J. and Gonos E.S. (2003) Central role of the proteasome in senescence and survival of human fibroblasts: induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation. J. Biol. Chem. 278: 28026–28037
Chondrogianni N., Petropoulos I., Franceschi C., Friguet B., and Gonos E.S. (2000) Fibroblast cultures from healthy centenarians have an active proteasome. Exp. Gerontol. 35: 721-728

2^nd Axis: Proteasome activation and its effects in cellular and organismal models of ageing and Alzheimer's disease

Previous work has shown that proteasome activation is feasible in the cellular level or in the organismal level (C. elegans) either through genetic means or through compounds. Compound-mediated proteasome activation occurs through stereochemical interaction with the proteolytic proteasome core, through post-translational modifications of the proteasome subunits or regulators or through the activation of transcription factors that regulate the expression of the proteasome subunits under specific conditions such as the Nuclear factor (erythroid-derived 2)-like 2) (Nrf2). Proteasome activation results in cellular and organismal lifespan extension and healthspan amelioration.
Ageing is a major risk factor for age-related diseases including proteinopathies such as Alzheimer’s (AD), Parkinson’s (PD) and Huntington’s (HD) diseases. Moreover, the proteasome has been shown to malfunction during the progression of all these diseases. We have shown that proteasome activation results in deceleration of the progression of the AD and HD phenotype in the relative nematode models. We have also revealed that proteasome activation results in reduced Aβ-mediated toxicity in human cells of neuronal origin as well as in murine cortical neurons.
Consequently, we are interested in revealing the effects and the involved mechanisms of proteasome activation on the progression of ageing and various proteinopathies mainly focusing on AD.

Related Publications

Vasilopoulou MA., Ioannou E., Roussis V. and Chondrogianni N. (2021) Modulation of the ubiquitin-proteasome system by marine natural products. Redox Biol. 41:101897
Kapetanou M., Chondrogianni N.,* Petrakis S., Koliakos G. and Efstathios S. Gonos (2017) Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells. Free Rad. Biol. Med. 103:226-235. (* co-corresponding author)
Papaevgeniou N., Sakellari M., Jha S., Tavernarakis N., Holmberg C.I., Gonos E.S and Chondrogianni N. (2016) 18α- glycyrrhetinic acid proteasome activator decelerates aging and Alzheimer's disease progression in C. elegans and neuronal cultures. Antiox. Redox Signal. 25: 855-869. (cover page)
Chondrogianni N.,* Georgila K., Kourtis N., Tavernarakis N., Gonos E.S. (2015) 20S proteasome activation promotes life span extension and resistance to proteotoxicity in Caenorhabditis elegans. FASEB J 29: 611-622.(* co-corresponding author)
Graikou K., Kapeta S., Aligiannis N., Sotiroudis G., Chondrogianni N., Gonos E.S. and Chinou I. (2011) Chemical Analysis of Greek Pollen - Antioxidant, antimicrobial and proteasome activation properties. Chem Cent J. 5: 33
Chondrogianni N.,* Kapeta S., Chinou I., Vassilatou K., Papassideri I. and Gonos E.S. (2010) Anti-ageing and rejuvenating effects of quercetin. Exp. Gerontol. 45: 763-771. (* co-corresponding author)
Kapeta S., Chondrogianni N.* and Gonos E.S. (2010) Νrf2 mediated proteasome activation delays senescence in human fibroblasts. J. Biol. Chem. 285: 8171-8184.(* co-corresponding author)
Chondrogianni N. and Gonos E.S. (2007) Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence. Exp. Gerontol. 42: 899-903
Katsiki M., Trougakos I.P., Chondrogianni N., Alexopoulos E.C., Makropoulos V. and Gonos E.S. (2004) Αlterations of senescence biomarkers in human cells by exposure to CrVI in vivo and in vitro. Exp. Gerontol.39:1079-1087
Chondrogianni N., Tzavelas C., Pemberton A.J., Nezis I.P., Rivett A.J. and Gonos E.S. (2005) Overexpression of proteasome beta 5 subunit increases the amount of assembled proteasome and confers ameliorated response to oxidative stress and higher survival rates. J. Biol.Chem. 280: 11840-11850

3^rd Axis: Screening and identification of compounds with preventive/therapeutic properties against ageing and/or Alzheimer's disease

We are working with a very powerful model organism, the nematode worm Caenorhabditis elegans. This is a great model for the identification of compounds with anti-ageing properties in a significantly short period. Moreover, there are several nematode strains serving as models for various proteinopathies, including Alzheimer’s disease. Using this model organism and the relative strains, we perform screening to identify compounds with anti-ageing and anti-aggregation properties that may ultimately be used in preventive or therapeutic strategies against ageing and/or Alzheimer's disease. Our results are usually verified in existing cellular models of ageing (replicative senescence model) or of the respective disease.

Related Publications

Delivoria D.C., Chia S., Habchi J., Perni M., Matis I., Papaevgeniou N., Reczko M., Chondrogianni N., Dobson C.M., Vendruscolo M., Skretas G. (2019) Bacterial production and direct functional screening of expanded molecular libraries for discovering inhibitors of protein aggregation. Sci Adv. 5(10): eaax5108.
Matis I., Delivoria D.C., Mavroidi B., Papaevgeniou N., Panoutsou S., Bellou S., Papavasileiou K.D., Linardaki Z.I., Stavropoulou A.V., Vekrellis K., Boukos N., Kolisis F.N., Gonos E.S., Margarity M., Papadopoulos M.G., Efthimiopoulos S., Pelecanou M., Chondrogianni N., Skretas G. (2017) An integrated bacterial system for the discovery of chemical rescuers of disease-associated protein misfolding. Nature Biomed. Eng. 1: 838–852.
Filippopoulou K., Papaevgeniou N., Lefaki M., Paraskevopoulou A., Biedermann D, Kren V. and Chondrogianni N. (2017) 2,3-Dehydrosilybin A/B as a pro-longevity and anti-aggregation compound. Free Rad. Biol. Med. 103:256-267.
Koufaki M.,* Fotopoulou T., Kapetanou M., Heropoulos GA*, Gonos E.S, Chondrogianni N*. (2014) Microwave-assisted synthesis of 3,5-disubstituted isoxazoles and evaluation of their anti-ageing activity. Eur. J. Med. Chem. 83: 508-515. (* equal contribution)

4^th Axis: Nrf2 in ageing and stress

The Nuclear factor (erythroid-derived 2)-like 2) (Nrf2) transcription factor is the major factor orchestrating the antioxidant cellular response. Various proteasome subunits are included among its target genes. Previous work has identified 18α-glycyrrhetinic acid as a natural proteasome activator that works through the activation of Nrf2, leading to cellular (human primary fibroblasts) and lifespan (C. elegans) extension. We therefore investigate the role of Nrf2 in the progression of ageing and in stress response, mainly (but not exclusively) focusing on its role on proteasome regulation.

Related Publications

Lefaki M., Papaevgeniou N., Tur J.A., Vorgias C.E., Sykiotis G.P., Chondrogianni N. (2020) The dietary triterpenoid 18α-Glycyrrhetinic acid protects from MMC-induced genotoxicity through the ERK/Nrf2 pathway. Redox Biol. 28: 101317.
Kapeta S., Chondrogianni N.* and Gonos E.S. (2010) Νrf2 mediated proteasome activation delays senescence in human fibroblasts. J. Biol. Chem. 285: 8171-8184.(* co-corresponding author)

5^th Axis: Toxicity testing of drugs or novel bioactive compounds in the model of the nematode C. elegans

The nematode worm Caenorhabditis elegans is an emerging model in biomedical and environmental toxicology. It is well-validated for toxicological research and recommended by NIH for whole-animal toxicology and safety testing. This is mainly based on the following advantages of the organism: (1) the chemical effects on C. elegans predict toxic effects in mammals, (2) testing on C. elegans reduces the use of mammals in toxicology assays, (3) the extended knowledge on C. elegans physiology, coupled with powerful gene manipulation technologies, provide powerful insights into toxic effects on the level of a whole organism and uncover the complex interactions between the genes and the environment. A standardized soil toxicological testing method using C. elegans has already been adopted by the American Society for Testing and Materials (ASTM, 2002) and later by the International Standards Organization in Europe (ISO 2007). Moreover, C. elegans is well suited for neurotoxicological studies. Phenotypic characteristics such as feeding behavior, developmental timing, fecundity and motility have been shown to be affected upon exposures to heavy metals, pesticides, genotoxic agents as well as due to proteotoxicity during the progression of proteinopathies. Consequently, its use permits the toxicity assessment of compounds. We have optimized all the necessary protocols in the lab and may provide a fast toxicity testing of drugs or novel bioactive compounds using this multicellular organism. We also perform further testing in human primary fibroblasts.

Related Publications

Magoulas GE., Kalopetridou L., Ćirić A., Kritsi E., Kouka P., Zoumpoulakis P., Chondrogianni N., Soković M., Prousis KC., Calogeropoulou T. (2020) Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents. Bioorganic Chem. 106: 104509.

Honors & Awards

Award of best oral presentation, 9th International Conference on Oxidative Stress in Skin Biology and Medicine, Online event, 2021 (Dr. A. Gioran, Post-doctoral fellow)
Award of publication with highest impact factor and SNIP (first and last name from ICB/NHRF) of the host Institute (ICB) in 2020: Lefaki M., Papaevgeniou N., Tur J.A., Vorgias C.E., Sykiotis G.P., Chondrogianni N. (2020) The dietary triterpenoid 18α-Glycyrrhetinic acid protects from MMC-induced genotoxicity through the ERK/Nrf2 pathway. Redox Biol. 28: 101317.
Award of publication with highest impact factor (first and last name from ICB/NHRF) of the host Institute (ICB) in 2019: Delivoria, D.C., Chia, S., Habchi, J., Perni, M., Matis, I., Papaevgeniou, N., Reczko, M., Chondrogianni, N., Dobson, C.M., Vendruscolo, M., Skretas, G.* (2019). Bacterial production and direct functional screening of expanded molecular libraries for discovering inhibitors of protein aggregation. Sci. Adv. 5.
Award of best poster presentation, 70th National Conference of Hellenic Society of Biochemistry and Molecular Biology, Athens, Greece, 2019 (Dr. D. Delivoria, we were co-investigators in this work)
Award of poster presentation, The Society of Free Radical Research - Europe 2018 Annual Conference, Lisbon, Portugal, 2018 (Ms N. Papaevgeniou, PhD student)
Award of 2nd best poster communication, The Society of Free Radical Research - Europe 2016 Annual Conference, Budapest, Hungary, 2016 (Mr D. Draganidis, we were co-investigators in this work)
Award of best poster communication, EMBO Conference ”Molecular chaperones: From molecules to cells and misfolding diseases”, Crete, Greece, 2015 (Dr. G. Skretas, we were co-investigators in this work)
Award of best poster communication, The Society of Free Radical Research - Europe 2014 Summer School, Spetses, Greece, 2014 (Ms M. Lefaki, PhD student)
Award of best oral communication, 64th National Conference of Hellenic Society of Biochemistry and Molecular Biology, Athens, Greece, 2013 (Ms K. Georgila, Research associate)
Award of best poster communication, The Society of Free Radical Research - Europe 2013 Annual Conference, Athens, Greece, 2013 (Ms K. Georgila, Research associate)
7th Catherine Pasquier Award 2011, SFRR-Europe meeting, Istanbul, Turkey, 2011 (Dr. N. Chondrogianni)
Spetsai International Young Investigator Award, 40 Years' Spetsai Summer School Anniversary, “Workshop on Molecular and Cell Biology at Spetsai: Past, Present and Future - A Forty Years' Anniversary”, Spetses, Greece, 2006 (Dr. N. Chondrogianni, Post-doctoral fellow)
Award of best oral presentation, 7th Annual Meeting of Hellenic Research Club for Connective Tissue and Matrix Biology, Patras, Greece, 2005 (Dr. N. Chondrogianni, Post-doctoral fellow)
Award of Young Scientists “Gardiki-Kouidou” 2003 (Ph. D. student), 55th Meeting of Hellenic Society of Biochemistry and Molecular Biology, Athens, Greece, 2003 (Ms N. Chondrogianni, PhD student)
Award of poster presentation “Cancer and Ageing award”, The Society of Free Radical Research - Europe 2003 Annual Conference, Ioannina, Greece, 2003 (Ms N. Chondrogianni, PhD student)

Patents

Koufaki M., Calogeropoulou T., Chondrogianni N., Papahatjis D., Gonos E.S., Fotopoulou T., Prousis K., and Chatzapi E. (2018) “Bio-inspired proteasome activators with anti-aging properties”
a. National patent (2018-0100-094 [OBI-Industrial Property Organisation])
b. PCT/GR2019/000018
Gonos E.S., Chondrogianni N. and Chinou I. (2009) “Anti-aging properties of quercetin, 18α-glycyrrhetinic acid, hederagenin and quercetin caprylate”
a. International (EP2246037A2) patent
b. National patent (2009-0100-242 [OBI-Industrial Property Organisation])
(accompanied by cooperation agreement for the commercial exploitation of the patent with Korres®)
Relative product: ''Quercetin and Oak'' (a series of anti-ageing and anti-wrinkles products) and “Black Pine” by Korres Natural Products

Education & Training

Lectures in Master of Science Program “Oncology: from Oncogenesis to Therapy”, University of Crete - School of Medicine and The National Hellenic Research, 2019-present
Lectures in Master of Science Program “Bioentrepreneurism”, Department of Biochemistry and Biotechnology, University of Thessaly and Institute of Chemical Biology, The National Hellenic Research Foundation, 2018-present
Lectures in the Master of Science Program “Cosmetology-Dermopharmacology”, Department of Pharmacy, National and Kapodistrian University of Athens, 2019-present
Lectures in Master of Science Program “Molecular and Applied Physiology”, Department of Medicine, National and Kapodistrian University of Athens, 2012-2019
Lectures in Master of Science Program “Biological Applications in Medicine”, Department of Biology, National and Kapodistrian University of Athens, 2012-2014
Lectures in Master of Science Program “Bioinformatics”, Department of Biology, National and Kapodistrian University of Athens, Molecular Biology and Genomics Course 2011-2021
Lectures in Master of Science Program “Educational Biology and Novel technologies”, Department of Biology, National and Kapodistrian University of Athens, Biology II Course 2011-2017
Lecture in Master of Science Program in Biology (Master BIO I), University of Namur, FUNDP/URBC, Namur, Belgium, 2009, “Proteasome and Cellular Ageing”
Laboratory courses:
- Undergraduate students (Department of Biology, National and Kapodistrian University of Athens, 2002-2004)
- Postgraduate students (Master of Science Program “Biological Applications in Medicine”, Department of Biology, National and Kapodistrian University of Athens, 2002-2014)

Science & Society

Anti-ageing and youth: How can constituents found in our normal diet help us?
"Youth, Antiaging and Longevity: Modern Approaches to Biological Age through the Historical and Biological Sciences", March 2018, Science and Society annual event of the NHRF aiming at communicating front line issues to the general public.
Presentation of the group and the field of ageing to students visiting NHRF.

Group Structure and Personnel

Dr. Niki Chondrogianni, Research Director, Group Leader

Current lab members

Post-doctoral fellows
Dr. Anna Gioran

PhD students
Eleni Panagiotidou
Sofia Lagoumtzi

Master thesis students
Athina Soulioti

Collaborating University Faculty and Researchers
Dr. Efstathios S. Gonos (Dec 2021-present)
Prof. Vassilios Roussis (2020-present)

Former lab members

Post-doctoral fellows
Dr. Ioannis Ninios
Dr. Paraskevi Kouka
Dr. Margarita Theodoropoulou

PhD students
Maria Lefaki
Nikoletta Papaevgeniou
Mary Vasilopoulou
Giorgos Paikopoulos
Dimitra Ranti

Research associates
Fotini Aktypi

Master thesis students
Mirella Sakellari
Nikoletta Papaevgeniou
Konstantina Philippopoulou
Anna Paraskeuopoulou
Efthimia Giosa
Fotini Aktypi
Ioannis Foukas (bibliographical research master thesis)

Bsc thesis students
Apostolos Petsas

Trainees/Visitors
Justyna Sobich
Dimitris Draganidis
Fotini Aktipi
Antonio Pellín Carcelén

Lab supervision

BSc thesis students
Anna Gioran

Trainees/Visitors
Dr. Valerie Vanhooren
Dr. Kosara Smiljanic

Collaborations

Former and/or current collaborations with Universities / Research Institutes outside NHRF

National

Prof. L. Alexopoulos, National Technical University of Athens
Dr. A. Chatziioannou, Biomedical Research Foundation, Academy of Athens
Prof. C. Demetzos, National and Kapodistrian University of Athens
Prof. I. Fatouros, University of Thessaly
Prof. G. Garinis, Institute of Molecular Biology and Biotechnology (IMBB), Crete
Prof. E. Ioannou, National and Kapodistrian University of Athens
Dr. D. Kletsas, NCSR “Demokritos”
Dr. S. Petrakis, Center for Research and Technology Hellas
Prof. V. Roussis, National and Kapodistrian University of Athens
Dr. P. Syntichaki, Biomedical Research Foundation, Academy of Athens
Prof. N. Tavernarakis, Institute of Molecular Biology and Biotechnology (IMBB), Crete
Dr. I. Zoidakis, Biomedical Research Foundation, Academy of Athens

International

Dr. D. Bano, DZNE, Germany
Prof. B. Friguet, Sorbonne University, France
Dr. T. Grune, DIfE German Institute of Human Nutrition Potsdam-Rehbruecke, Germany
Dr. C. Holmberg, University of Helsinki, Finland
Prof. B. Karademir, Marmara University, Turkey
Prof. V. Kren, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Dr. A. Ori, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Germany
Prof. I. Petropoulos, Sorbonne University, France
Dr. E. Pick, University of Haifa, Israel
Prof. A. Simm, Martin Luther University Halle- Wittenberg, Germany
Prof. A. Vasilaki, University of Liverpool, UK

Companies

The collaboration of our group with companies occurs either through common funded projects or the provision of services to reveal anti-ageing properties of specific products or to determine ageing markers.

Boderm Pharmaceutical S.A.
DHI Athens
Intermed Pharmaceutical Laboratories
Korres Natural Products

Funding

Funded Research Projects

Proteostasis “Proteasome activation: Understanding of the systemic regulation of proteostasis during the progression of ageing and identification of anti-ageing structural proteasome activators from the Mediterranean Sea” (MIS 5050341, coordinator: Dr. N. Chondrogianni), Operational Programme “Human Resources Development, Education and Lifelong Learning 2014-2020” under the call “Research support with an emphasis on young researchers”, 2020-2021, Total/Lab Budget: 45.500 €, Role: Coordinator-Principal Investigator
Algosmetic “Isolation and characterization of metabolites with anti-aging, wound-healing and melanogenesis-inhibitory properties” (project code: Τ6ΥВΠ-00474, coordinator: Prof. E. Ioannou), Operational Program “Competitiveness, Entrepreneurship and Innovation” under the call “Special Actions Aquaculture – Industrial Materials – Open Innovation in Culture”, 2019-2021, Lab budget: 45.000 €, Role: Principal Investigator
ProMiDis ERC Consolidator Grant “A unified drug discovery platform for protein misfolding diseases” (coordinator: Dr. G. Skretas), European Research Council (ERC) Consolidator Grant, 2019-2024, Lab budget: To be defined. Role: Workgroup member/co-supervisor of task 6 (C. elegans)
PEPtADe “Preclinical studies of pharmacological interest on potent inhibitors of the Alzheimer’s Disease” (project code: T1EDK-00353, coordinator: Prof. V. Ikonomidou), Operational Program “Competitiveness, Entrepreneurship and Innovation”, under the call “Research-Create-Innovate”, 2018-2021, Lab budget: 210.000 €, Role: Principal Investigator
RESET “Bioinspired antiaging proteasome activators” (project code: T1EDK-01610, coordinator: INTERMED), Operational Program “Competitiveness, Entrepreneurship and Innovation”, under the call “Research-Create-Innovate”, 2018-2021, Lab budget: 170.665 €, Role: Principal Investigator/Member of NHRF Workgroup
STHENOS-β “Targeted therapeutic approaches against degenerative diseases, with emphasis on cancer and aging- Optimization of bioactive compounds” (MIS 5002398, coordinator: Dr. A. Pintzas), Developmental Projects of Research Organisations- Kripis, Institute of Biology, Medicinal Chemistry and Biotechnology, 2017-2020, Lab budget: 22.000 €, Role: Partner
Empirikion Foundation Scientific Projects 2010 (initiation during 2013) “Increasing intracellular anti-oxidant cellular capacity during ageing: the role of Nrf1 and Nrf2 transcription factors and the molecular cross-talk with the proteasome” (coordinator: Dr. N. Chondrogianni), 2013-2016, Lab budget: 18.000 €, Role: Coordinator-Principal Investigator
Latsis Scientific Projects 2013 “Using bacteria and worms in the battle against Alzheimer’s disease” (coordinator: Dr. N. Chondrogianni), 2013, Total/Lab budget: 12.000 €, Role: Coordinator-Principal Investigator
STHENOS Program “KRIPIS” “Targeted therapeutic approaches against degenerative diseases, with emphasis on cancer and aging”, Institute of Biology, Medicinal Chemistry and Biotechnology, 2013-2015, Lab budget: 22.000 €, Role: Partner
StemAge “Revealing strategies with antioxidant/antiageing properties in human stem cells” (coordinator: Prof. G. Koliakos) Program “Cooperation” 2013-2015, Lab budget: 120.000 €, Role: Member of NHRF Workgroup
MAESTRO “Modelling the dynamic progression of cellular aging through the development of systems biology and bioinformatics tools” (coordinator: Prof. A. Bezerianos) Ministry of Education, Lifelong Learning and Religious Affairs-Program “Thalis”, 2012-2015, Lab budget: 132.500 €, Role: Principal Investigator
GEnAge «The role of Genetic and Environmental Factors in Aging and Longevity» (coordinator: Dr. G. Garinis) Ministry of Education, Lifelong Learning and Religious Affairs-Program “Thalis”, 2012-2015, Lab budget: 71.400 €, Role: Principal Investigator
Degrade-Age “Integrative research on the molecular role of proteolytic mechanisms on proteome maintenance and stability during the progression of ageing and age-related diseases.” (Greek coordinator: Dr. N. Chondrogianni, German coordinator: Dr. D. Bano) Programme for the promotion of the exchange and scientific cooperation between Greece and Germany – Programme IKYDA 2012, 2012-2014, Lab budget: 10.000 €, Role: Coordinator-Principal Investigator
CMST COST Action CM1001 “Chemistry of non-enzymatic protein modification – modulation of protein structure and function” (coordinator: Dr. T. Grune) (MC Substitute Member), 2010-2014, Lab budget: Depending on the training activities and the meetings to be attended.
BMBS COST Action BM1203 “EU-ROS” (coordinator: Prof. A. Daiber) (MC Substitute Member), 2012-2015, Lab budget: Depending on the training activities and the meetings to be attended.
BMBS COST Action BM1307 “PROTEOSTASIS” (coordinator: Dr. R. Barrio) (MC Member), 2014-2018, Lab budget: Depending on the training activities and the meetings to be attended
BMBS COST Action BM1408 “GENiE” (coordinator: Dr. S. Jarriault) (MC Member), 2015-2019, Lab budget: Depending on the training activities and the meetings to be attended
CA COST Action 16112 “NutRedOx” (coordinator: Prof. M. Cherkaoui Malki) (MC Member), 2017-2021, Lab budget: Depending on the training activities and the meetings to be attended
CA COST Action 20113 “ProteoCure” “A sound proteome for a sound body: targeting proteolysis for proteome remodeling” (coordinator: Dr. R. Farras) (MC Member), 2021-2025, Lab budget: Depending on the training activities and the meetings to be attended
CA COST Action 20121 “BenBedPhar” “Bench to bedside transition for pharmacological regulation of Nrf2 in non-communicable diseases” (coordinator: Prof. A. Cuadrado) (MC Member), 2021-2025, Lab budget: Depending on the training activities and the meetings to be attended

Funded projects from the industry

DHI “Analysis of carbonylated proteins in hair follicles of donors of different ages” (2017-2018) (coordinator: Dr. N. Chondrogianni) Total/Lab budget: 19.115 €, Role: Coordinator-Principal Investigator

Scholarships to members of the group (PhD projects peer reviewed)

Ms Dimitra Ranti, “Study of the effects of inducible proteasome activation in ageing and Alzheimer’s disease progression in the nematode C. elegans”, 2022-2024, Hellenic Foundation of Research and Innovation (HFRI)/PhD fellowships, declined by the fellow to move abroad
Ms Eleni Panagiotidou, “Study of the induction effects of proteostasis mechanisms in aging and age-related diseases”, 10/2019- 3/2022, Hellenic Foundation of Research and Innovation (HFRI)/PhD fellowships
Mrs Eleni Panagiotidou, “Study of the induction effects of proteostasis mechanisms in aging and age-related diseases”, 2019, Empirikion Foundation Scholarships for PhD students, declined by the Fellow to accept the HFRI scholarship
Ms Nikoletta Papaevgeniou, “Study of the effects of lipofuscin and AGEs in the progression of aging”, 12/2016-05/2018, Empirikion Foundation Scholarships for PhD students

Publications

Research articles in peer-reviewed journals

Draganidis D., Jamurtas AZ., Chondrogianni N., Mastorakos G., Jung T., Grune T., Papadopoulos C., Papanikolaou K., Papassotiriou I., Papaevgeniou N., Poulios A., Batrakoulis, A., Deli CK., Georgakouli K., Chatzinikolaou A., Karagounis LG., Fatouros IG. (2021) Low-grade systemic inflammation interferes with anabolic and catabolic characteristics of the aged human skeletal muscle. Oxid. Med.Cell. Longev. 8376915: 1-14.
Aktypi F., Papaevgeniou N., Voutetakis C., Chatziioannou A., Grune T., Chondrogianni N. (2021) Network analysis in aged C. elegans reveals candidate regulatory genes of ageing. Biogerontology 22: 345-367.
Magoulas GE., Kalopetridou L., Ćirić A., Kritsi E., Kouka P., Zoumpoulakis P., Chondrogianni N., Soković M., Prousis KC., Calogeropoulou T. (2020) Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents. Bioorganic Chem. 106: 104509.
Sacramento Kelmer E., Kirkpatrick J.M., Mazzetto M., Baumgart M., Bartolome A., Di Sanzo S., Caterino C., Sanguanini M., Papaevgeniou N., Lefaki M., Childs D., Bagnoli S., Terzibasi Tozzini E., Di Fraia D., Romanov N., Sudmant P., Huber W., Chondrogianni N., Vendruscolo M., Cellerino A. and Ori A. (2020) Reduced proteasome activity in the aging brain results in ribosome stoichiometry loss and aggregation. Mol. Syst Biol 16(6):e9596. (cover page)
Laidou S., Alanis-Lobato G., Pribyl J., Raskó T., Tichy B., Mikulasek K., Tsagiopoulou M., Oppelt J., Kastrinaki G., Lefaki M., Singh M., Zink A., Chondrogianni N., Psomopoulos F., Prigione A., Ivics Z., Pospisilova S., Skladal P., Izsvák Z., Andrade-Navarro M.A., Petrakis S. (2020) Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery. Redox Biology 32: 101458.
Binenbaum I., Lefaki M., Chondrogianni N.*,Chatziioannou A. (2020) Bioinformatic framework for analysis of transcription factor changes as the molecular link between replicative cellular senescence signaling pathways and carcinogenesis. Biogerontology 21: 357-366.(* co-corresponding author)
Lefaki M., Papaevgeniou N., Tur J.A., Vorgias C.E., Sykiotis G.P., Chondrogianni N. (2020) The dietary triterpenoid 18α-Glycyrrhetinic acid protects from MMC-induced genotoxicity through the ERK/Nrf2 pathway. Redox Biol. 28: 101317.
Papaevgeniou N., Hoehn A., Tur J.A., Klotz L.O., Grune T., Chondrogianni N. (2019) Sugar-derived AGEs accelerate pharyngeal pumping rate and increase the lifespan of Caenorhabditis elegans. Free Radic Res. 13: 1-12.
Delivoria D.C., Chia S., Habchi J., Perni M., Matis I., Papaevgeniou N., Reczko M., Chondrogianni N., Dobson C.M., Vendruscolo M., Skretas G. (2019) Bacterial production and direct functional screening of expanded molecular libraries for discovering inhibitors of protein aggregation. Sci Adv. 5(10): eaax5108.
Sakellari M., Chondrogianni N.*, Gonos E.S. (2019) Protein synthesis inhibition induces proteasome assembly and function. Biochem Biophys Res Commun. 514: 224-230. (* co-corresponding author)
Draganidis D., Jamurtas A.Z., Stampoulis T., Laschou V., Deli C.K., Georgakouli K., Papanikolaou K., Chatzinikolaou A., Michalopoulou M., Papadopoulos C., Tsimeas P., Chondrogianni N., Koutedakis Y., Karagounis L.G. and Fatouros I.G. (2018) Disparate habitual physical activity and dietary intake profiles of elderly men with low and elevated systemic inflammation. Nutrients 10 (5), pii: E566.
Athanasopoulou S., Chondrogianni N., Santoro A., Asimaki K., Delitsikou V., Voutetakis K., Fabbri C., Pietruszka B., Kaluza J., Franceschi C., Gonos E.S. (2018) Beneficial effects of elderly tailored Mediterranean diet on the proteasomal proteolysis. Front. Physiol. 9: 457.
Matis I., Delivoria D.C., Mavroidi B., Papaevgeniou N., Panoutsou S., Bellou S., Papavasileiou K.D., Linardaki Z.I., Stavropoulou A.V., Vekrellis K., Boukos N., Kolisis F.N., Gonos E.S., Margarity M., Papadopoulos M.G., Efthimiopoulos S., Pelecanou M., Chondrogianni N., Skretas G. (2017) An integrated bacterial system for the discovery of chemical rescuers of disease-associated protein misfolding. Nature Biomed. Eng. 1: 838–852.
Draganidis D., Chondrogianni N., Chatzinikolaou A., Terzis G., Karagounis L.G., Sovatzidis A., Avloniti A., Lefaki M., Protopappa M., Deli C.K., Papanikolaou K., Jamurtas A.Z., Fatouros I.G. (2017) Protein ingestion preserves proteasome activity during intense aseptic inflammation and facilitates skeletal muscle recovery in humans. Br. J. Nutr. 118: 189-200.
Filippopoulou K., Papaevgeniou N., Lefaki M., Paraskevopoulou A., Biedermann D, Kren V. and Chondrogianni N. (2017) 2,3-Dehydrosilybin A/B as a pro-longevity and anti-aggregation compound. Free Rad. Biol. Med. 103:256-267.
Kapetanou M., Chondrogianni N.*, Petrakis S., Koliakos G. and Efstathios S. Gonos (2017) Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells. Free Rad. Biol. Med. 103:226-235. (* co-corresponding author)
Papaevgeniou N., Sakellari M., Jha S., Tavernarakis N., Holmberg C.I., Gonos E.S and Chondrogianni N. (2016) 18α- glycyrrhetinic acid proteasome activator decelerates aging and Alzheimer's disease progression in C. elegans and neuronal cultures. Antiox. Redox Signal. 25: 855-869. (cover page)
Chondrogianni N.*, Georgila K., Kourtis N., Tavernarakis N., Gonos E.S. (2015) 20S proteasome activation promotes life span extension and resistance to proteotoxicity in Caenorhabditis elegans. FASEB J 29: 611-622.(* co-corresponding author)
Koufaki M*., Fotopoulou T., Kapetanou M., Heropoulos GA*, Gonos E.S, Chondrogianni N*. (2014) Microwave-assisted synthesis of 3,5-disubstituted isoxazoles and evaluation of their anti-ageing activity. Eur. J. Med. Chem. 83: 508-515. (* equal contribution)
Tsolou Α., Nelson G.; Trachana V., Chondrogianni N., Saretzki G., von Zglinicki T.; Gonos E.S. (2012) The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult. IUBMB Life 64: 432-442.
Graikou K., Kapeta S., Aligiannis N., Sotiroudis G., Chondrogianni N., Gonos E.S. and Chinou I. (2011) Chemical Analysis of Greek Pollen - Antioxidant, antimicrobial and proteasome activation properties. Chem Cent J. 5: 33.
Chondrogianni N.*, Kapeta S., Chinou I., Vassilatou K., Papassideri I. and Gonos E.S. (2010) Anti-ageing and rejuvenating effects of quercetin. Exp. Gerontol. 45: 763-771. (* co-corresponding author)
Kapeta S., Chondrogianni N.* and Gonos E.S. (2010) Νrf2 mediated proteasome activation delays senescence in human fibroblasts. J. Biol. Chem. 285: 8171-8184.(* co-corresponding author)
Trougakos I.P., Chondrogianni N., Amarantos I., Blake J., Schwager C., Ansorge W. and Gonos E.S. (2010) Genome-wide transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines. Cancer Genet Cytogenet. 196: 109-118.
Catalgol B., Ziaja I., Breusing N., Jung T., Hoehn A., Alpertunga B., Schroeder P., Chondrogianni N., Gonos E.S., Petropoulos I., Friguet B., Klotz L.O., Krutmann J. and Grune T. (2009) The proteasome is an integral part of solar ultraviolet a radiation-induced gene expression. J. Biol. Chem. 284: 30076-30086.
Balantinou E., Trougakos I.P., Chondrogianni N., Margaritis L.H. and Gonos E.S. (2009) Transcriptional and post-translational regulation of clusterin by the two main cellular proteolytic pathways. Free Rad. Biol. Med. 46:1267-1274.
Chondrogianni N., Trougakos I.P., Kletsas D., Chen Q.M. and Gonos E.S. (2008) Partial proteasome inhibition in human fibroblasts triggers accelerated M1 senescence or M2 crisis depending on p53 and Rb status. Aging Cell,7: 717-732. (journal’s highlight)
Poulios E., Trougakos I.P., Chondrogianni N. and Gonos E.S. (2007) Exposure of human diploid fibroblasts to hypoxia extends proliferative lifespan. Ann. N. Y. Acad. Sci. 1119: 9-19.
Chondrogianni N. and Gonos E.S. (2007) Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence. Exp. Gerontol. 42: 899-903.
Katsiki M., Chondrogianni N., Chinou I., Rivett A.J., and Gonos E.S. (2007) The olive constituent oleuropein exhibits proteasome stimulatory properties in vitro and confers lifespan extension of human embryonic fibroblasts. Rejuvenation Res. 10: 157-172.
Stratford F.L., Chondrogianni N., Trougakos I.P., Gonos E.S. and Rivett A.J. (2006) Proteasome response to interferon-gamma is altered in senescent human fibroblasts. FEBS Lett. 580: 3989-3994.
Chondrogianni N., Tzavelas C., Pemberton A.J., Nezis I.P., Rivett A.J. and Gonos E.S. (2005) Overexpression of proteasome beta 5 subunit increases the amount of assembled proteasome and confers ameliorated response to oxidative stress and higher survival rates. J. Biol.Chem. 280: 11840-11850.
Chondrogianni N., Simoes D.C.M., Francheschi C. and Gonos E.S. (2004) Cloning of differentially expressed genes in skin fibroblasts from centenarians. Biogerontology 5: 401-409.
Katsiki M., Trougakos I.P., Chondrogianni N., Alexopoulos E.C., Makropoulos V. and Gonos E.S. (2004) Αlterations of senescence biomarkers in human cells by exposure to CrVI in vivo and in vitro. Exp. Gerontol.39:1079-1087.
Chondrogianni N. and Gonos E.S. (2004) Proteasome inhibition induces a senescence-like phenotype in primary human fibroblasts cultures. Biogerontology 5: 55-61.
Chondrogianni N., Stratford F.L.L., Trougakos I.P., Friguet B., Rivett A.J. and Gonos E.S. (2003) Central role of the proteasome in senescence and survival of human fibroblasts: induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation. J. Biol. Chem. 278: 28026–28037.
Agiostratidou G., Sgouros I., Galani A., Voulgari A., Chondrogianni N., Samantas E., Demopoulos M.A., Skarlos D. and Gonos E.S. (2001) Correlation of in vitro cytoxicity and clinical response to therapy in ovarian and breast cancer patients. Anticancer Res. 21: 455-459.
Chondrogianni N., Petropoulos I., Franceschi C., Friguet B., and Gonos E.S. (2000) Fibroblast cultures from healthy centenarians have an active proteasome. Exp. Gerontol. 35: 721-728.

Reviews in peer-reviewed journals

Gkekas I., Gioran A., Boziki M.K., Grigoriadis N., Chondrogianni N. and Petrakis S. (2021) Oxidative stress and neurodegeneration: interconnected processes in polyQ diseases. Antioxidants 10, 1450.
Lagoumtzi S. and Chondrogianni N. (2021) Senolytics and Senomorphics: natural and synthetic therapeutics in the treatment of aging and chronic diseases. Free Radical Biol. Med. 171: 169-190.
Vasilopoulou MA., Ioannou E., Roussis V. and Chondrogianni N. (2021) Modulation of the ubiquitin-proteasome system by marine natural products. Redox Biol. 41:101897.
Gioran A. and Chondrogianni N. (2020) Mitochondria (cross)talk with proteostatic mechanisms; focusing on ageing and neurodegenerative diseases. Mech. Ageing. Den. 190:111324.
Panagiotidou E. and Chondrogianni N. (2020) We are what we eat: ubiquitin-proteasome system (UPS) modulation through dietary products. Adv. Exp. Med. Biol. 1233: 329-348.
Paunkov A., Chartoumpekis DV., Ziros PG., Chondrogianni N., Kensler TW., Sykiotis GP. (2019) Impact of Antioxidant Natural Compounds on the Thyroid Gland and Implication of the Keap1/Nrf2 Signaling Pathway. Curr Pharm Des., 25: 1828-1846.
Santín-Márquez R., Alarcón-Aguilar A., López-Diazguerrero N.E., Chondrogianni N., Königsberg M. (2019) Sulforaphane - role in aging and neurodegeneration. Geroscience, 41: 655-670.
Papaevgeniou N. and Chondrogianni N. (2018) Identification of anti-aging and anti-aggregation polyphenolic compounds using C. elegans as a model organism. Curr Pharm Des. 24: 2107-2120.
Lefaki M., Papaevgeniou N. and Chondrogianni N. (2017) Redox regulation of proteasome function. Redox Biology 13: 452-458.
Egea J, Fabregat I, Frapart YM, Ghezzi P, Görlach A, Kietzmann T, Kubaichuk K, Knaus UG, Lopez MG, Olaso-Gonzalez G, Petry A, Schulz R, Vina J, Winyard P, Abbas K, Ademowo OS, Afonso CB, Andreadou I, Antelmann H, Antunes F, Aslan M, Bachschmid MM, Barbosa RM, Belousov V, Berndt C, Bernlohr D, Bertrán E, Bindoli A, Bottari SP, Brito PM, Carrara G, Casas AI, Chatzi A, Chondrogianni N, Conrad M, Cooke MS, Costa JG, Cuadrado A, My-Chan Dang P, De Smet B, Debelec-Butuner B, Dias IHK, Dunn JD, Edson AJ, El Assar M, El-Benna J, Ferdinandy P, Fernandes AS, Fladmark KE, Förstermann U, Giniatullin R, Giricz Z, Görbe A, Griffiths H, Hampl V, Hanf A, Herget J, Hernansanz-Agustín P, Hillion M, Huang J, Ilikay S, Jansen-Dürr P, Jaquet V, Joles JA, Kalyanaraman B, Kaminskyy D, Karbaschi M, Kleanthous M, Klotz LO, Korac B, Korkmaz KS, Koziel R, Kračun D, Krause KH, Křen V, Krieg T, Laranjinha J, Lazou A, Li H, Martínez-Ruiz A, Matsui R, McBean GJ, Meredith SP, Messens J, Miguel V, Mikhed Y, Milisav I, Milković L, Miranda-Vizuete A, Mojović M, Monsalve M, Mouthuy PA, Mulvey J, Münzel T, Muzykantov V, Nguyen ITN, Oelze M, Oliveira NG, Palmeira CM, Papaevgeniou N, Pavićević A, Pedre B, Peyrot F, Phylactides M, Pircalabioru GG, Pitt AR, Poulsen HE, Prieto I, Rigobello MP, Robledinos-Antón N, Rodríguez-Mañas L, Rolo AP, Rousset F, Ruskovska T, Saraiva N, Sasson S, Schröder K, Semen K, Seredenina T, Shakirzyanova A, Smith GL, Soldati T, Sousa BC, Spickett CM, Stancic A, Stasia MJ, Steinbrenner H, Stepanić V, Steven S, Tokatlidis K, Tuncay E, Turan B, Ursini F, Vacek J, Vajnerova O, Valentová K, Van Breusegem F, Varisli L, Veal EA, Yalçın AS, Yelisyeyeva O, Žarković N, Zatloukalová M, Zielonka J, Touyz RM, Papapetropoulos A, Grune T, Lamas S, Schmidt HHHW, Di Lisa F, Daiber A. (2017) European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biol. 13: 94-162.
Chondrogianni N., Voutetakis K., Kapetanou M., Delitsikou V., Papaevgeniou N., Sakellari M., Lefaki M., Filippopoulou K. and Gonos E.S. (2015) Proteasome activation: An innovative promising approach for delaying aging and retarding age-related diseases. Ageing Res. Rev. 23 (Pt A): 37-55.(co-corresponding author)
Chondrogianni N., Sakellari M., Lefaki M., Papaevgeniou N., Gonos E.S. (2014) Proteasome activation delays aging in vitro and in vivo. Free Rad. Biol. Med. 71: 303-320.(corresponding author)
Papaevgeniou N., Chondrogianni N. (2014) The ubiquitin proteasome system in Caenorhabditis elegans and its regulation. Redox Biol. 2: 333-347.
Chondrogianni N., Petropoulos I., Grimm S., Georgila K., Catalgol B., Friguet B., Grune T., Gonos E.S. (2014) Protein damage, repair and proteolysis. Mol. Aspects Med. 35: 1-71.(co-corresponding author)
Aldini G, Vistoli G, Stefek M, Chondrogianni N, Grune T, Sereikaite J, Sadowska-Bartosz I, Bartosz G. (2013) Molecular strategies to prevent, inhibit and degrade advanced glycoxidation and advanced lipoxidation end products. Free Radic Res. 47 Suppl 1:93-137.
Chondrogianni N. and Gonos E.S. (2010) Proteasome function determines cellular homeostasis and the rate of aging. Adv. Exp. Med. Biol. 694: 38-46.
Chondrogianni N. and Gonos E.S. (2008) Proteasome activation as a novel anti-aging strategy. IUBMB Life 60: 651-655.
Chondrogianni N. and Gonos E.S. (2005) Proteasome dysfunction and mammalian aging: involved steps and factors. Exp. Gerontol. 40: 931-938.
Chondrogianni N., Fragoulis E.S. and Gonos E.S. (2002) Protein degradation during aging: the lysosome-, the calpain- and the proteasome-dependent cellular proteolytic systems. Biogerontology 3: 121-123.
Friguet B., Bulteau A-L., Chondrogianni N., Conconi M. and Petropoulos I. (2000) Protein degradation by the proteasome and its implication in ageing. Ann. N. Y. Acad. Sci. 908: 143-154.
Petropoulou C., Chondrogianni N., Simoes D., Agiostratidou G., Drosopoulos N., Kotsota V. and Gonos E.S. (2000) Ageing and longevity: A paradigm of complementation between genetic control and failure of homeostasis? Ann. N. Y. Acad. Sci. 908: 133-142.

Editor in books

Editor in Chondrogianni N., Pick E. and Gioran A (Eds) Proteostasis and Proteolysis: A volume in the Oxidative Stress and Disease book series, CRC Press, Taylor & Francis Group, 2021.

Chapters in books

Chountoulesi, M., Naziris, N., Gioran, A., Papagiannopoulos, A., Steele, B., Micha-Screttas, M., Stavrinides, S., Hanias, M., Chondrogianni, N.,* Pispas, S.,* Arbez-Gindre, C.,* Demetzos, C. (2022) Applications of Nanotechnology in Alzheimer’s Disease. In Vlamos P., Kotsireas I.S. and Tarnanas I. (Eds) Handbook of Computational Neurodegeneration, Meteor(* co-corresponding author)
Vasilopoulou M.A, Papaevgeniou N. and Chondrogianni N. (2021) Proteasome fate in aging and proteinopathies. In Chondrogianni N., Pick E. and Gioran A (Eds) Proteostasis and Proteolysis: A volume in the Oxidative Stress and Disease book series, CRC Press, Taylor & Francis Group. Accepted
Ranti D., Gioran A. and Chondrogianni N. (2021) Autophagy in aging and oxidative stress. In Chondrogianni N., Pick E. and Gioran A (Eds) Proteostasis and Proteolysis: A volume in the Oxidative Stress and Disease book series, CRC Press, Taylor & Francis Group. Accepted
Chondrogianni N., Vasilopoulou M.A., Kapetanou M., Gonos E.S. (2020) Proteasome Modulation: A Way to Delay Aging? In: Rattan, S.I.S. (Ed.), Encyclopedia of Biomedical Gerontology. Elsevier. vol. 3, Academic Press, pp. 92–104.
Papaevgeniou N. and Chondrogianni N. (2016) UPS activation in the battle against aging and aggregation-related diseases: An extended review. In: Rune Matthiesen (ed.), Proteostasis: Methods and Protocols, Methods in Molecular Biology, vol. 1449, pp.1-70 Springer Science+Business Media New York, USA
Chondrogianni N. and Gonos E.S. (2012) The Proteasomal System in Aging and Disease: Structure and Function of the Ubiquitin-Proteasome System: Modulation of components. In Grune’s Progress in Molecular Biology and Translational Science (M. Conn, ed.) vol. 109, pp. 41–74 Elsevier, USA.
Gonos E.S. Trougakos I.P., and Chondrogianni N. (2008) Aging Research in Greece. In: Palmore, E., Whittington, F. and Kunkel, S. (eds): The International Handbook of Aging: current research and development. pp 249-257, Praeger Publishers, Connecticut, USA.
Chondrogianni N., Chinou I. and Gonos E.S. (2008) Anti-aging properties of the olive constituent oleuropein in human cells. In: V.R. Preedy and R.R. Watson (eds): Olives and olive oil in Health and Disease Prevention. Oxford: Academic Press, 2010, pp. 1335-1343.
Trougakos I.P., Chondrogianni N., Pimenidou A., Katsiki M., Tzavelas C. and Gonos E.S. (2003) Slowing down cellular ageing in vitro. S.I.S. Rattan (ed): Modulating Aging and Longevity 65-83 Kluwer Academic Publishers.

Editorials

Pfirrmann T., Chondrogianni N., Olzscha H., Vasilaki A. (2021) Dysregulated Protein Homeostasis in the Aging Organism, Frontiers in Molecular Biosciences Special Issue
Chondrogianni N., Pick E., Gioran A. (2021) Preface. Chondrogianni N., Pick E. and Gioran A (Eds) Proteostasis and Proteolysis: A volume in the Oxidative Stress and Disease book series, CRC Press, Taylor & Francis Group.
Gonos E.S., Chondrogianni N., Djordjevic A.M. (2019) Where ageing goes nowadays: mechanisms, pathways, biomarkers and anti-ageing strategies. Mech.Ageing and Dev. 177: 1-3.
Gonos E.S., Trougakos I.P. and Chondrogianni N. (2007) Preface. Ann. N. Y. Acad. Sci. 1119: xi-xii.

Monographs

Chondrogianni N. (2004) PhD thesis. Study of the molecular mechanisms of regulation of ageing and longevity. Department of Biology, National and Kapodistrian University of Athens.

Conference Proceedings

Proceedings of International Conferences

Vasilopoulou M.A., Fotopoulou T., Papaevgeniou N., Gioran A., Papahatjis D., Calogeropoulou T., Koufaki M. and Chondrogianni N. (2021) A novel bioinspired proteasome activator against aging. Free Radical Biology and Medicine 177: S122-S123.
Gioran Α. and Chondrogianni N. (2021) Proteasome activation in C. elegans engages UPRmt. Free Radical Biology and Medicine 177: S64.
Gkikas G., Katsiris D., Kostaki M., Ladopoulos G., Ieronymaki D., Theodoraki E., Ioannidou V., Vitsos A., Chondrogianni N., Gioran A., Sfiniadakis I., Papaioannou G.T., Rallis M. (2021) Skin carcinogenesis mouse models. Free Radical Biology and Medicine 177: S113.
Papaevgeniou N., Fotopoulou T., Vasilopoulou M., Panat N., Pick E., Golan A., Gonos E.S., Papahatjis D., Calogeropoulou T., Koufaki M., Chondrogianni N. (2019) A novel bioinspired proteasome activator: potential anti-ageing strategies offered by mother nature (but not only). Free Radic Biol Med. 139 Suppl 1: S78-S79
Kirkpatrick J.M., Sacramento E.K., Mazzetto M., Di Sanzo S., Caterino C., Bartolome A., Sanguanini M., Papaevgeniou N., Lefaki M., Childs D., Terzibasi-Tozzini E., Romanov N., Baumgart M., Huber W., Chondrogianni N., Vendruscolo M., Cellerino A., Ori A. (2019) Reduced proteasome activity in the aging brain results in ribosome stoichiometry loss and aggregation. Mol. Cell. Proteomics 18: S35
Papaevgeniou N., Panagiotidou E., Filippopoulou K., Chondrogianni N. (2019) Quest for bioactive compounds in our diet with anti-ageing and anti-aggregation properties. Proceedings 11: 34, Proceedings of Natural Products and the Hallmarks of Chronic Diseases—COST Action 16112, Luxemburg
Papaevgeniou N., Hoehn A., Grune T., Chondrogianni N. (2018) Lipofuscin-mediated alterations effects on organismal ageing. Free Radic Biol Med. 120 Suppl 1: S78-S79. Proceedings of the 19th Biennial Meeting of the Society for Free Radical Research International (SFRRI), Lisbon, Portugal
Chondrogianni N. (2017) Proteasome activators as diet constituents: a promising strategy against aging and aggregation. Free Radic Biol Med. 108 Suppl 1: S6-S7. Proceedings of the OCC World Congress / Annual SFRR-E Conference Location: Berlin, Germany
Papaevgeniou N., Hoehn A., Grune T., Chondrogianni N. (2017) Lipofuscin effects in Caenorhabditis elegans ageing model. Free Radic Biol Med. 108 Suppl 1: S48-S48. Proceedings of the OCC World Congress / Annual SFRR-E Conference Location: Berlin, Germany
Fatouros I.G., Draganidis D., Chondrogianni N., Jamurtas A.Z., Chatzinikolaou A., Terzis G., Sovatzidis A., Protopappa M., Papanikolaou K., Avloniti A. (2016).Protein supplementation alters redox-status and proteosomic activity following aseptic inflammation induced by exercise. Free Radic Biol Med. 96 Suppl 1: S42-S42. Proceedings of the Conference of the Society-for-Free-Radical-Research-Europe (SFRR-E) Location: Budapest, Hungary
Chondrogianni N. (2015) The pivotal role of proteasome enhancement in ageing, longevity and proteostasis across species. Free Radic Biol Med. 86 Suppl 1: S12-S12. Proceedings of the SFRR-E/SNFS Conference Location: Stuttgart, Germany
Lefaki M., Chatzinikolaou G., Andoniade C., Garinis G., Chondrogianni N. (2015) Investigation of the crosstalk between proteasome function and nucleotide excision repair mechanism during ageing. Free Radic Biol Med. 86 Suppl 1: S5-S6. Proceedings of the SFRR-E/SNFS Conference Location: Stuttgart, Germany
Georgila K.; Chondrogianni N.; Kourtis N.; Tavernarakis N, Gonos E.S. (2014) Enhanced proteasome degradation extends Caenorhabditis elegans lifespan and ameliorates neurodegeneration. FEBS J. 281 Suppl 1: 243. Proceedings of the FEBS EMBO 2014 Conference, Paris, France
Kapetanou M., Chondrogianni, N., Gonos S. (2014) Study of the role of the proteasome in senescence of stem cells. FEBS J. 281 Suppl 1: 249, Proceedings of the FEBS EMBO 2014 Conference, Paris, France
Georgila K, Voutetakis K, Delitsikou V, Chondrogianni N, Gonos ES. (2014) Optimization of in vitro measurement of proteasome activity in mammalian cells using fluorogenic substrates. Free Radic Biol Med. 75 Suppl 1:S31. Proceedings of the SFRR-E 2014 Conference, Paris, France
Chondrogianni N, Georgila K, Kourtis N, Tavernarakis N, Gonos Efstathios S. (2014) Enhanced proteasome degradation extends Caenorhabditis elegans lifespan and alleviates aggregation-related pathologies. Free Radic Biol Med. 75 Suppl 1:S18. Proceedings of the SFRR-E 2014 Conference, Paris, France
Skretas G., Delivoria D., Matis I., Papaevgeniou N., Chondrogianni N. (2014) Engineering bacteria for the discovery of potential therapeutic compounds against protein misfolding diseases. New Biotech. 31, Suppl S: S12-S13. Proceedings of the 16th European Congress on Biotechnology, 2013, Edinburgh, United Kingdom.
Aldini G., Chondrogianni N., Grune T., Sadowska-Bartosz I., Sereikaite J., Stefek M., Vistoli G., Bartoszn G. (2013) Strategies to prevent post-translational protein modifications. Free Radic Biol Med. 65 Suppl 1:S11. Proceedings of the SFRR-E 2013 Conference, Athens, Greece
Georgila K., Chondrogianni N., Kourtis N., Tavernarakis N. and Gonos E. (2013) Enhanced proteasome degradation extends Caenorhabditis elegans lifespan and ameliorates neurodegeneration. Free Radic Biol Med. 65 Suppl 1:S32. Proceedings of the SFRR-E 2013 Conference, Athens, Greece
Papaevgeniou N., Sakellari M., Gonos E.S. and Chondrogianni N. (2013) Study of the effects of natural compounds in the lifespan of the nematode Caenorhabditis elegans. Free Radic Biol Med. 65 Suppl 1:S41. Proceedings of the SFRR-E 2013 Conference, Athens, Greece
Zampeli V., Shakibaei M., Makrantonaki E., Tzellos T., Fimmel S., Chondrogianni N., Gonos ES., Zouboulis C.C. (2011) Involvement of fibroblasts in adipose tissue development. Exp. Dermatology 20: 165-166. Proceedings of the 38th Annual Meeting of the Arbeitsgemeinschaft-Dermatologishche-Forschung, Tuebingen, Germany
Kapeta S., Chondrogianni N., Sisoula C., Gonos E.S. (2010) Proteasomal activation by Nrf2 extends lifespan in human fibroblasts. FEBS J. 277 Suppl 1 :285. Proceedings of the 35th Congress of the Federation-of-European-Biochemical-Societies, Gothenburg, Sweden
Balantinou E., Trougakos I.P., Chondrogianni N., Gonos E.S. (2009) Transcriptional and post-translational regulation of clusterin/apolipoprotein J by the two main cellular proteolytic pathways. FEBS J. 276: 358-359, Proceedings of the 34th Congress of the Federation-of-European-Biochemical-Societies Location: Prague, Czech Republic
Chondrogianni N., Chinou I., Vassilatou K, Gonos E.S. (2009) Identification of natural compounds that promote proteasome activation and confer lifespan extension. PLANTA MEDICA 75: 940. Proceedings of the 55th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-Research-and-Natural-Product-Research, Geneva, Switzerland
Catalgol B., Ziaja I., Breusing N., Jung T., Hohn A., Alpertunga B., Schroeder P., Chondrogianni N., Gonos E.S., Petropoulos I., Friguet B., Klotz L.O., Grune T., Krutmann J. (2009) The proteasome is an integral part of the signaling cascade leading to solar UVA-induced gene expression. J Invest. Dermatology 129: S129. Proceedings of the 69th Annual Meeting of the Society-of-Investigative-Dermatology, Montreal, Canada
Graikou K., Aligiannis N., Chondrogianni N., Kapeta S., Gonos E.S., Chinou I. (2008) Chemical analysis and anti-aging properties of Greek pollen. PLANTA MEDICA 74: 1101-1102. Proceedings of the 7th Joint Meeting of the Association-Francophone-pour-l'Enselgnement-et-la-Recherche-en-Pharmacognosie/American-Society-of-Pharmacognosy/Society-for-Medicinal-Plant-Research/Phytochem-Society-of-Europe/Societa-Italiana-di-Fitochimica, Athens, Greece
Balantinou E., Trougakos I.P., Chondrogianni N., Gonos E.S. (2008) Multiple levels of clusterin regulation following proteasome and lysosome inhibition. FEBS J 275 Suppl 1: 208. Proceedings of the Joint Conference of the 33rd FEBS Congress/11th IUBMB Conference Location: Athens, Greece
Kapeta S., Chondrogianni N., Gonos, E.S. (2008) Identification of natural compounds that promote proteasome activation and induce lifespan extension. FEBS J 275 Suppl 1: 209. Proceedings of the Joint Conference of the 33rd FEBS Congress/11th IUBMB Conference Location: Athens, Greece
Chondrogianni N., Trougakos I.P., Kletsas D., Gonos E.S. (2008) Partial proteasome inhibition triggers p53-dependent premature senescence. FEBS J 275 Suppl 1: 445. Proceedings of the Joint Conference of the 33rd FEBS Congress/11th IUBMB Conference Location: Athens, Greece
Chondrogianni N., Trougakos I.P., Gonos E.S. (2007) The role of proteasome manipulation on cellular senescence, stress response and longevity. FEBS J 274 Suppl 1: 219. Proceedings of the 32nd Congress of the Federation-of-European-Biochemical-Societies (FEBS), Vienna, Austria
Chondrogianni N., Gonos E.S. (2006) The effects of proteasome manipulation in ageing, survival and longevity. FEBS J 273 Suppl 1: 308. Proceedings of the 31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) Location: Istanbul, Turkey
Chondrogianni N., Gonos E.S. (2005) Overexpression of proteasome beta 5 subunit increases amount of assembled proteasome and confers ameliorated response to oxidative stress and higher survival rates. FEBS J 272 Suppl 1: 151. Proceedings of the IUBMB 50th Anniversary Symposium Location: Budapest, Hungary
Chondrogianni N., Trougakos I.P., Stratford F.L.L., Friguet B., Rivett A.J., Gonos E.S. (2003) Central role of the proteasome in senescence and survival of human fibroblasts. Free Radic Res 37 Suppl 1: 112. Proceedings of the Meeting on Free Radicals and Oxidative Stress Location: Ioannina, Greece

Proceedings of National Conferences

Filippopoulou K., Papaevgeniou N., Papadodima O., Pilalis E., Hamodrakas S., Chatziioannou A. and Chondrogianni N. (2014) Βioinformatics analysis of a set of transcription microarray data related to the ageing and Alzheimer’s disease progression using Caenorhabditis elegans as a model system, Proceedings of the 9th conference of the Hellenic Society for Computational Biology & Bioinformatics HSCBB14 –Athens, Greece