The NHRF_ICB Flow Cytometry Facility is located in Room Y22 of the Institute of Chemical Biology, 48 Vassileos Constantinou Ave.11635, Athens Greece
The Facility provides access to Flow cytometry instrumentation, expertise and training allowing the analysis of single cells or particles (bacteria, yeast, beads etc.).
Flow cytometry can be used for phenotypic analysis of cells, for the analysis of apoptosis, cell cycle, DNA content, cytokine production and many other Flow cytometry applications.
Measurements are extremely rapid – up to 70,000 particles per second with up to 7 fluorescent parameters plus 2 scatter parameters analyzed on each cell.
The Facility also provides flow cytometry data analysis for accurate quantification of all parameters of interest.
Fluorescent Activated Cell Sorting (FACS) allows the physical sorting of cells of interest for further downstream analysis (functional assays, cloning, culture, transcriptomics, proteomics or microscopic analysis).
Instrumentation
FACS ARIA II (BD)
Multicolour high-speed cell sorter, equipped with 2 lasers (488, 633 nm) and 7 detectors to measure up to 7 fluorescent parameters. The instrument is capable of the simultaneous sorting of four populations into tubes or plates.
Cyflow ML (Partec)
1 laser analyzer (488), capable of measuring up to 3 fluorescent parameters.
Research collaborations | Services to external users
On hands training for novice and intermediate users on the independent use of flow cytometry analyzer Cyflow ML, and post-acquisition data analysis.
Training and guidance on all aspects of experimental design for flow cytometry experiments.
Fully operated cell sorting is offered following consultation with the Facility manager (pstamou@eie.gr). Single cell sorting, cell type enrichment and high purity bulk sorts are available.
For any of the above collaborations/services please contact the Facility manager Dr Panagiota Stamou.
